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Am J Physiol Lung Cell Mol Physiol 260: L179-L188, 1991;
1040-0605/91 $5.00
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AJP - Lung Cellular and Molecular Physiology, Vol 260, Issue 4 179-L188, Copyright © 1991 by American Physiological Society

ARTICLES

Quantitative approaches for studying gene expression

R. J. Wiesner and R. Zak
Department of Medicine, University of Chicago, Illinois 60637.

The methods currently available for measuring mRNAs and proteins are reviewed, with a special emphasis on their application to physiological questions. The article focuses on the quantitative determination of cellular contents, but also on assessment of rates of synthesis and degradation, and turnover.


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