AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 261: L249-L254, 1991;
1040-0605/91 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Das, S. K.
Right arrow Articles by Fanburg, B. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Das, S. K.
Right arrow Articles by Fanburg, B. L.

AJP - Lung Cellular and Molecular Physiology, Vol 261, Issue 4 249-L254, Copyright © 1991 by American Physiological Society

ARTICLES

TGF-beta 1 produces a "prooxidant" effect on bovine pulmonary artery endothelial cells in culture

S. K. Das and B. L. Fanburg
Department of Medicine, New England Medical Center, Boston, Massachusetts 02111.

The pulmonary endothelium is known to be sensitive to oxidant injury, including that of hyperoxia. Similar to effects of exposure to 80-95% O2, porcine platelet transforming growth factor (TGF)-beta 1 at concentrations of greater than or equal to 0.3 ng/ml inhibited proliferation and caused enlargement of bovine pulmonary artery endothelial cells after 24 h of incubation in room air. Uptake of [3H]thymidine, but not of [3H]deoxycytidine, was suppressed by both hyperoxia and TGF-beta 1. The cellular enlargement produced by TGF-beta 1 in room air was attenuated in the presence of anoxia, indicating a need for O2 for TGF-beta 1 to have an effect on cell size. In the presence of 20 microM FeCl3, both TGF-beta 1 and 80% O2 produced marked cellular desquamation from culture dishes. The antioxidants dimethyl sulfoxide and vitamin E partially counteracted the growth inhibitory effect of TGF-beta 1 on endothelial cells. In contrast to its effect on endothelial cells, TGF-beta 1 only moderately altered size and proliferation of smooth muscle cells from the same pulmonary vessels. Uptake of [3H]thymidine by smooth muscle cells was uninfluenced in 48 h by TGF-beta 1, and little, if any, desquamation of these cells occurred with TGF-beta 1 in the presence of 20 microM FeCl3. We propose from these experiments that TGF-beta 1 may produce an oxidant effect on vascular endothelium that is capable of causing injury to this tissue.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R.-M. Liu, Y. Liu, H. J. Forman, M. Olman, and M. M. Tarpey
Glutathione regulates transforming growth factor-{beta}-stimulated collagen production in fibroblasts
Am J Physiol Lung Cell Mol Physiol, January 1, 2004; 286(1): L121 - L128.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. Tadlock, Y. Yamagiwa, J. Hawker, C. Marienfeld, and T. Patel
Transforming growth factor-{beta} inhibition of proteasomal activity: a potential mechanism of growth arrest
Am J Physiol Cell Physiol, August 1, 2003; 285(2): C277 - C285.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. M. Factor, A. Kiss, J. T. Woitach, P. J. Wirth, and S. S. Thorgeirsson
Disruption of Redox Homeostasis in the Transforming Growth Factor-alpha /c-myc Transgenic Mouse Model of Accelerated Hepatocarcinogenesis
J. Biol. Chem., June 19, 1998; 273(25): 15846 - 15853.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online