AJP - Lung Cellular and Molecular Physiology, Vol 263, Issue 6 723-L726, Copyright © 1992 by American Physiological Society

ARTICLES

Effect of an anti-Mo1 MAb on ozone-induced airway inflammation and airway hyperresponsiveness in dogs

Z. Li, E. E. Daniel, C. G. Lane, M. A. Arnaout and P. M. O'Byrne
Department of Biomedical Science, McMaster University, Hamilton, Ontario, Canada.

Ozone inhalation causes neutrophil migration into the airway and airway hyperresponsiveness in dogs. The leukocyte adhesion molecule Mo1 (CD11b/CD18) is a heterodimeric glycoprotein the expression of which is necessary for neutrophil adhesion to endothelium. To evaluate the contribution of Mo1 to ozone-induced neutrophil influx and airway hyperresponsiveness, six dogs were treated intravenously with an Anti-Mo1 monoclonal antibody (3.75 mg/kg in normal saline) that binds to both human and canine Mo1, or the diluent alone, 1.5 h before inhaling ozone (3 ppm for 30 min), or dry air. Airway responses to doubling doses of inhaled acetylcholine (ACh) were measured before and after inhalation of ozone. Neutrophil influx was assessed by bronchoalveolar lavage (BAL) performed after the second ACh inhalation. Treatment with anti-Mo1 prevented the ozone-induced influx of neutrophils into BAL. After diluent and inhaled dry air, the neutrophil count in BAL was 1.49 +/- 1.26 (SE) x 10(4) (5.0% of total cells). After diluent and inhaled ozone, the neutrophil count increased to 7.27 +/- 3.22 (SE) x 10(4) (22.6% of total cells) (P < 0.05). After anti-Mo1 and inhaled ozone, the neutrophil count was 1.48 +/- 0.62 (SE) x 10(4) (8.5% of total cells). Treatment with anti-Mo1 also significantly reduced the number of eosinophils in BAL after ozone. Ozone-induced ACh airway hyperresponsiveness was not prevented by treatment with anti-Mo1. These results indicate that expression of Mo1 is necessary for ozone-induced neutrophil migration into the airway lumen.

This article has been cited by other articles:

				S. A. Shore Obesity and asthma: lessons from animal models J Appl Physiol, February 1, 2007; 102(2): 516 - 528. [Abstract] [Full Text] [PDF]

				B. L. Yost, G. J. Gleich, D. B. Jacoby, and A. D. Fryer The changing role of eosinophils in long-term hyperreactivity following a single ozone exposure Am J Physiol Lung Cell Mol Physiol, October 1, 2005; 289(4): L627 - L635. [Abstract] [Full Text] [PDF]

				R. A. Johnston, J. P. Mizgerd, and S. A. Shore CXCR2 is essential for maximal neutrophil recruitment and methacholine responsiveness after ozone exposure Am J Physiol Lung Cell Mol Physiol, January 1, 2005; 288(1): L61 - L67. [Abstract] [Full Text] [PDF]

				S. A. SHORE, I. N. SCHWARTZMAN, B. LE BLANC, G. G. KRISHNA MURTHY, and C. M. DOERSCHUK Tumor Necrosis Factor Receptor 2 Contributes to Ozone-induced Airway Hyperresponsiveness in Mice Am. J. Respir. Crit. Care Med., August 15, 2001; 164(4): 602 - 607. [Abstract] [Full Text] [PDF]

				D. M. Hyde, L. A. Miller, R. J. McDonald, M. Y. Stovall, V. Wong, K. E. Pinkerton, C. D. Wegner, R. Rothlein, and C. G. Plopper Neutrophils enhance clearance of necrotic epithelial cells in ozone-induced lung injury in rhesus monkeys Am J Physiol Lung Cell Mol Physiol, December 1, 1999; 277(6): L1190 - L1198. [Abstract] [Full Text] [PDF]

				A. N. Freed, R. Cueto, and W. A. Pryor Antioxidant transport modulates peripheral airway reactivity and inflammation during ozone exposure J Appl Physiol, November 1, 1999; 87(5): 1595 - 1603. [Abstract] [Full Text] [PDF]

				H. KOTO, M. SALMON, E.-B. HADDAD, T.-J. HUANG, J. ZAGORSKI, and K. CHUNG Role of Cytokine-induced Neutrophil Chemoattractant (CINC) in Ozone-induced Airway Inflammation and Hyperresponsiveness Am. J. Respir. Crit. Care Med., July 1, 1997; 156(1): 234 - 239. [Abstract] [Full Text]

				A. IGARASHI, H. IIJIMA, G. TAMURA, and K. SHIRATO Tazanolast Inhibits Ozone-induced Airway Hyperresponsiveness in Guinea Pigs Am. J. Respir. Crit. Care Med., May 1, 1997; 157(5): 1531 - 1535. [Abstract] [Full Text]