AJP - Lung Cellular and Molecular Physiology, Vol 264, Issue 1 66-L73, Copyright © 1993 by American Physiological Society

ARTICLES

ED1-containing cellular fibronectin release into lung lymph during lung vascular injury with postoperative bacteremia

T. Rizk, R. Rebres, P. Vincent, E. Lewis, P. McKeown-Longo and T. M. Saba
Department of Physiology and Cell Biology, Albany Medical College of Union University, New York 12208.

Fibronectin (Fn) exists in both a soluble and insoluble form. Soluble Fn in plasma and lymph is an opsonic molecule that enhances phagocytic host defense. Insoluble Fn in the subendothelial and extracellular matrix is an adhesive molecule that mediates cell adhesion to substratum. The extracellular matrix of tissues such as the lung contains a mixture of both plasma-derived fibronectin (pFn) as well as locally synthesized cellular fibronectin (cFn). cFn is antigenically related to pFn, but cFn has extra domains (ED1 and ED2) that do not exist in liver synthesized pFn. The purpose of this study was to determine whether ED1-Fn was released into lung lymph before an increase in lung vascular permeability following postoperative bacteremia. Male sheep (n = 8) with surgically prepared lung lymph fistulae were infused intravenously with a sublethal dose (5 x 10(8)) of Pseudomonas aeruginosa 2 days following surgery. Lymph flow (QL), lymph-to-plasma (L/P) total protein ratio, lung protein clearance (QL x L/P), and hemodynamics were measured over 48 h following bacterial challenge. The lymph and plasma ED1-Fn concentrations were determined by enzyme-linked immunosorbent assay (ELISA) using a murine monoclonal antibody specific to the ED1 region of human cFn. There was a rapid rise of ED1-Fn flux in lung lymph which was evident 60 min after the start of bacterial infusion, resulting in a maximum three- to fourfold increase (P < 0.05) in this parameter. In contrast, the ED1-Fn concentration in plasma before bacterial infusion was less than lung lymph and it did not increase over the initial 6 h following bacterial infusion.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				A. Saren, R. Virkola, J. Hacker, and T. K. Korhonen The Cellular Form of Human Fibronectin as an Adhesion Target for the S Fimbriae of Meningitis-Associated Escherichia coli Infect. Immun., May 1, 1999; 67(5): 2671 - 2676. [Abstract] [Full Text] [PDF]

				T. M. Curtis, R. F. Rotundo, P. A. Vincent, P. J. McKeown-Longo, and T. M. Saba TNF-alpha -induced matrix Fn disruption and decreased endothelial integrity are independent of Fn proteolysis Am J Physiol Lung Cell Mol Physiol, July 1, 1998; 275(1): L126 - L138. [Abstract] [Full Text] [PDF]

				T. P. Brien, P. P. Reddy, P. A. Vincent, E. P. Lewis, J. S. Ross, and T. M. Saba Lung matrix deposition of normal and alkylated plasma fibronectin: response to postsurgical sepsis Am J Physiol Lung Cell Mol Physiol, March 1, 1998; 274(3): L432 - L443. [Abstract] [Full Text] [PDF]