AJP - Lung Cellular and Molecular Physiology, Vol 264, Issue 2 100-L106, Copyright © 1993 by American Physiological Society

ARTICLES

Increased expression of PDGF A- and B-chain genes in rat lungs with hypoxic pulmonary hypertension

D. Katayose, M. Ohe, K. Yamauchi, M. Ogata, K. Shirato, H. Fujita, S. Shibahara and T. Takishima
First Department of Internal Medicine, Tohoku University School of Medicine, Miyagi, Japan.

To study the molecular basis of vascular remodeling in pulmonary hypertension, we developed an experimental system in which male Sprague-Dawley rats were exposed to hypoxia for up to 3 wk. Both the right ventricular systolic pressure and gravimetric index for right ventricular hypertrophy were higher in rats exposed to hypoxia for 3 wk than those of age-matched control rats (P < 0.01), indicating that pulmonary hypertension was established under conditions used. To examine the possible involvement of platelet-derived growth factor (PDGF) in the pulmonary vascular remodeling caused by hypoxia, we cloned rat PDGF A- and B-chain cDNA and prepared specific cRNA probes. Northern blot analysis revealed that PDGF B-chain mRNA levels in the lungs were increased, reached a maximum of day 1, and were sustained at day 3, whereas PDGF A-chain mRNA levels reached a maximum on day 3. Thus the increase in the PDGF B-chain mRNA level precedes that in the PDGF A-chain mRNA level. These results suggest that the PDGF A- and B-chain products may be coordinately and sequentially involved in hypoxic pulmonary vascular remodeling.

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