AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 264: L329-L337, 1993;
1040-0605/93 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by De Leyn, P.
Right arrow Articles by Flameng, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by De Leyn, P.
Right arrow Articles by Flameng, W.

AJP - Lung Cellular and Molecular Physiology, Vol 264, Issue 4 329-L337, Copyright © 1993 by American Physiological Society

ARTICLES

Adenine nucleotide degradation in ischemic rabbit lung tissue

P. De Leyn, T. Lerut, H. Schreinemakers, H. van Belle, J. Lauwerijns, F. van Lommel, E. Verbeken and W. Flameng
Division of Thoracic Surgery, Catholic University Leuven, Belgium.

The aim of the study was to determine the pathways and site of adenosine triphosphate (ATP) catabolism during lung ischemia, which thus far are largely unknown. For this purpose we used the isolated rabbit lung. Rabbit lungs were flushed in situ with a modified Krebs-Henseleit solution (60 ml/kg), the deflated heart lung blocks were isolated, immersed in saline solution, and stored at 37 degrees C. In group I (normothermic ischemia; n = 6) tissue content of ATP decreased progressively from 9.42 +/- 0.58 mumol/g dry wt to 3.42 +/- 0.24 mumol/g dry wt after 30 min of ischemia and further to 0.51 mumol/g dry weight after 4 h. Hypoxanthine was the major catabolite (92% of the nucleoside and purine base fraction at 4 h ischemia). Adenosine did not accumulate (preischemic 0.08 +/- 0.02 mumol/g dry weight vs. 0.13 +/- 0.01 mumol/g dry weight; P > 0.05). AMP accumulated, but also inosine monophosphate (IMP), which was undetectable before ischemia, increased significantly during ischemia. To determine the breakdown pathway of AMP, 400 microM of the adenosine deaminase inhibitor EHNA was added to the flush solution in group II (n = 6). During ischemia, ATP breakdown was unaltered but adenosine became the major catabolite (2.8 times the concentration of hypoxanthine at 4 h ischemia). By pretreatment of the rabbits with the nucleoside transport inhibitor R 75231 (group III; n = 6) no effect was observed on the concentrations during ischemia of inosine and hypoxanthine and only a minor increase of adenosine was found. Cytochemical localization of nucleoside phosphorylase revealed activity predominantly in the endothelial cells.(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
N. Fukuda, H. G. Folkesson, and M. A. Matthay
Relationship of interstitial fluid volume to alveolar fluid clearance in mice: ventilated vs. in situ studies
J Appl Physiol, August 1, 2000; 89(2): 672 - 679.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
Y. Minamiya, K. Tozawa, M. Kitamura, S. Saito, and J.-i. Ogawa
Platelet-activating Factor Mediates Intercellular Adhesion Molecule-1-dependent Radical Production in the Nonhypoxic Ischemia Rat Lung
Am. J. Respir. Cell Mol. Biol., July 1, 1998; 19(1): 150 - 157.
[Abstract] [Full Text]

Home page
 Ann. Thorac. Surg.Home page
D. E. M. Van Raemdonck, N. C. P. Jannis, F. R. L. Rega, P. R. J. De Leyn, W. J. Flameng, and T. E. Lerut
Delay of Adenosine Triphosphate Depletion and Hypoxanthine Formation in Rabbit Lung After Death
Ann. Thorac. Surg., July 1, 1996; 62(1): 233 - 240.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online