AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 264: L482-L489, 1993;
1040-0605/93 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jornot, L.
Right arrow Articles by Junod, A. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jornot, L.
Right arrow Articles by Junod, A. F.

AJP - Lung Cellular and Molecular Physiology, Vol 264, Issue 5 482-L489, Copyright © 1993 by American Physiological Society

ARTICLES

Variable glutathione levels and expression of antioxidant enzymes in human endothelial cells

L. Jornot and A. F. Junod
Respiratory Division, University Hospital, Geneva, Switzerland.

We have investigated the relationship between intracellular glutathione levels and the inducibility of the mRNAs encoding the major antioxidant enzymes Cu,Zn superoxide dismutase (Cu,Zn SOD), catalase (CAT), glutathione peroxidase (GP), and the stress protein heme oxygenase (HO) following exposure of human umbilical vein endothelial cells (HUVEC) to either hypoxanthine-xanthine oxidase or 95% O2. Treatment of HUVEC with 2 and 200 microM buthionine sulfoximine (BSO) for 16 h reduced total glutathione (GSH) levels by 51 and 95%, respectively, whereas treatment with 100 microM diethylmaleate (DEM) for 24 h increased the cellular GSH content by 58%. None of these treatments affected the responsiveness of HUVEC to a subsequent oxidant challenge, in terms of antioxidant enzymes activities and mRNA levels. On the contrary, HO mRNA was significantly induced by both BSO and DEM, as well as by hyperoxia, albeit to a different extent. We conclude that intracellular redox changes do not appear to regulate the expression of the mRNAs encoding Cu,Zn SOD, CAT, and GP. Furthermore, factors other than endogenous thiols may play a role in the control of HO mRNA expression.


This article has been cited by other articles:


Home page
 Eur Respir JHome page
C. Neurohr, A-G. Lenz, I. Ding, H. Leuchte, T. Kolbe, and J. Behr
Glutamate-cysteine ligase modulatory subunit in BAL alveolar macrophages of healthy smokers
Eur. Respir. J., July 1, 2003; 22(1): 82 - 87.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
I. Rahman and W. MacNee
Lung glutathione and oxidative stress: implications in cigarette smoke-induced airway disease
Am J Physiol Lung Cell Mol Physiol, December 1, 1999; 277(6): L1067 - L1088.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. E. Howlett, J. S. Hutchison, J. P. Veinot, A. Chiu, P. Merchant, and H. Fliss
Inhaled nitric oxide protects against hyperoxia-induced apoptosis in rat lungs
Am J Physiol Lung Cell Mol Physiol, September 1, 1999; 277(3): L596 - L605.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. M. Suttner, K. Sridhar, C. S. Lee, T. Tomura, T. N. Hansen, and P. A. Dennery
Protective effects of transient HO-1 overexpression on susceptibility to oxygen toxicity in lung cells
Am J Physiol Lung Cell Mol Physiol, March 1, 1999; 276(3): L443 - L451.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
B. Friedrichs, C. Muller, and R. Brigelius-Flohe
Inhibition of Tumor Necrosis Factor-{alpha}– and Interleukin-1–Induced Endothelial E-Selectin Expression by Thiol-Modifying Agents
Arterioscler. Thromb. Vasc. Biol., December 1, 1998; 18(12): 1829 - 1837.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
R. C. M. Siow, H. Sato, D. S. Leake, J. D. Pearson, S. Bannai, and G. E. Mann
Vitamin C Protects Human Arterial Smooth Muscle Cells Against Atherogenic Lipoproteins : Effects of Antioxidant Vitamins C and E on Oxidized LDL–Induced Adaptive Increases in Cystine Transport and Glutathione
Arterioscler. Thromb. Vasc. Biol., October 1, 1998; 18(10): 1662 - 1670.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
P. Pietarinen-Runtti, K. O. Raivio, M. Saksela, T. M. Asikainen, and V. L. Kinnula
Antioxidant Enzyme Regulation and Resistance to Oxidants of Human Bronchial Epithelial Cells Cultured under Hyperoxic Conditions
Am. J. Respir. Cell Mol. Biol., August 1, 1998; 19(2): 286 - 292.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C. M. Terry, J. A. Clikeman, J. R. Hoidal, and K. S. Callahan
Effect of tumor necrosis factor-alpha and interleukin-1alpha on heme oxygenase-1 expression in human endothelial cells
Am J Physiol Heart Circ Physiol, March 1, 1998; 274(3): H883 - H891.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. OH-ISHI, T. KIZAKI, T. OOKAWARA, T. SAKURAI, T. IZAWA, N. NAGATA, and H. OHNO
Endurance Training Improves the Resistance of Rat Diaphragm to Exercise-induced Oxidative Stress
Am. J. Respir. Crit. Care Med., November 1, 1997; 156(5): 1579 - 1585.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online