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Am J Physiol Lung Cell Mol Physiol 264: L504-L516, 1993;
1040-0605/93 $5.00
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AJP - Lung Cellular and Molecular Physiology, Vol 264, Issue 5 504-L516, Copyright © 1993 by American Physiological Society

ARTICLES

Fetal rat lung type II cell differentiation in serum-free isolated cell culture: modulation and inhibition

C. Fraslon, T. Lacaze-Masmonteil, V. Zupan, B. Chailley-Heu and J. R. Bourbon
Centre de Biologie Cellulaire, Centre National de la Recherche Scientifique, Ivry/Seine, France.

Undifferentiated fetal rat lung epithelial cells were isolated on gestational days 15 or 17 (term 22 days) and cultured in a defined medium. On plastic, most of the cells developed structurally abnormal lamellar bodies. On a basement membrane matrix (BMM), they sequentially accumulated glycogen and formed typical lamellar bodies. Biochemical analysis of the latter indicated that they had a phospholipid composition typical of surfactant for cells on BMM but not on plastic and that surfactant protein A appeared on BMM only. Progressing maturation from day 1 to day 6 in culture was demonstrated for 17-day cells on BMM by a sevenfold increase of labeled precursor incorporation into surfactant phospholipids. Exposure to medium conditioned by 21-day fetal fibroblasts enhanced incorporation already after a 1-day culture. The antisteroid RU 486 had no effect on differentiation, whereas transforming growth factor-beta, a factor produced by lung mesenchyme at early fetal stages, inhibited it markedly. Alveolar epithelial type II cells appear to be committed early, but their maturational process would be prevented until a definite gestational stage.


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