AJP - Lung Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 264: L523-L532, 1993;
1040-0605/93 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Murphy, J. K.
Right arrow Articles by Forman, H. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Murphy, J. K.
Right arrow Articles by Forman, H. J.

AJP - Lung Cellular and Molecular Physiology, Vol 264, Issue 5 523-L532, Copyright © 1993 by American Physiological Society

ARTICLES

Effects of sodium and proton pump activity on respiratory burst and pH regulation of rat alveolar macrophages

J. K. Murphy and H. J. Forman
Cell Biology Group of Childrens Hospital Los Angeles, California 90027.

At pH 7.4, extracellular Na+ removal inhibited the rat alveolar macrophage respiratory burst (RB) stimulated by phorbol 12-myristate 13-acetate (PMA) or zymosan-activated serum (ZAS). At pH 6.8, the RB was lower and decreased the Na+ effect. Amiloride inhibited the ZAS RB independently of effects on Na(+)-H+ exchange, but did not affect PMA stimulation. NBD-Cl, an H(+)-ATPase inhibitor, significantly inhibited the PMA or ZAS RB. Na+ removal caused sustained elevation of intracellular free [Ca2+], which previous studies suggested inhibits the RB. Intracellular pH (pHi) was lower at pHo 6.8 compared with pHo 7.4, but not altered by Na+ removal. PMA stimulation resulted in acidification corresponding with onset of superoxide production. At pHo 7.4, recovery to baseline pHi occurred that was not inhibited by amiloride or Na+ removal. In contrast, amiloride slowed pHi recovery after an exogenous acid load. Addition of H(+)-ATPase inhibitors, NBD-Cl or bafilomycin, following PMA stimulation or acid loading, inhibited pHi restoration. These studies suggest that pHi regulation following stimulation was mainly through a proton pump, whereas Na(+)-H+ exchange occurred only after greater acid loading. Nevertheless, Na+ and pH interacted to modulate the RB independent of Na(+)-H+ exchange.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
R. Liu, O. A. Carretero, Y. Ren, H. Wang, and J. L. Garvin
Intracellular pH regulates superoxide production by the macula densa
Am J Physiol Renal Physiol, September 1, 2008; 295(3): F851 - F856.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
T. E. Decoursey
Voltage-Gated Proton Channels and Other Proton Transfer Pathways
Physiol Rev, April 1, 2003; 83(2): 475 - 579.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
O. Ibrahim-Granet, B. Philippe, H. Boleti, E. Boisvieux-Ulrich, D. Grenet, M. Stern, and J. P. Latge
Phagocytosis and Intracellular Fate of Aspergillus fumigatus Conidia in Alveolar Macrophages
Infect. Immun., February 1, 2003; 71(2): 891 - 903.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Lowenthal and R. Levy
Essential Requirement of Cytosolic Phospholipase A2 for Activation of the H+ Channel in Phagocyte-like Cells
J. Biol. Chem., July 30, 1999; 274(31): 21603 - 21608.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. Eder
Ion channels in microglia (brain macrophages)
Am J Physiol Cell Physiol, August 1, 1998; 275(2): C327 - C342.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online