| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Lung Cellular and Molecular Physiology, Vol 265, Issue 1 53-L56, Copyright © 1993 by American Physiological Society
ARTICLES |
D. N. Cornfield, T. Stevens, I. F. McMurtry, S. H. Abman and D. M. Rodman
Department of Pediatrics, Children's Hospital, Denver Colorado.
We studied the effect of acute hypoxia on the cytosolic calcium concentration ([Ca2+]i) of fetal vascular smooth muscle cells (SMC) from late gestation fetal lambs. We tested the following hypotheses: 1) fetal pulmonary artery (PA) SMC sense hypoxia; 2) hypoxia stimulates transmembrane Ca2+ influx causing increased basal [Ca2+]i and Ca2+ responses to pharmacological vasoconstrictors; and 3) the response is unique to SMC from small (near resistance) PA. Fetal SMC were isolated from the proximal and distal pulmonary (DPA) and carotid arteries of late-gestation ovine fetuses, maintained in culture for 5-14 days prior to study, and studied with dual-excitation microfluorimetry using fura 2. Acute hypoxia caused a 233% increase in [Ca2+]i in distal PA SMC (P < 0.01), which was absent in low extracellular calcium bath. [Ca2+]i increased transiently in normoxic DPA SMC treated with angiotensin II, and oscillations in [Ca2+]i occurred (amplitude > or = 30 nM). In hypoxic DPA SMC the increase in [Ca2+]i was sustained and oscillations were attenuated or absent. [Ca2+]i in proximal PA SMC did not change with exposure to acute hypoxia and carotid artery SMC [Ca2+]i decreased by 13% (P < 0.05). We conclude that fetal SMC isolated from the DPA of late-gestation ovine fetuses directly sense decreased oxygen tension with an increase in [Ca2+]i that is dependent on the entry of extracellular Ca2+.
This article has been cited by other articles:
|
|
 |
|
  E. M. Whitman, S. Pisarcik, T. Luke, M. Fallon, J. Wang, J. T. Sylvester, G. L. Semenza, and L. A. Shimoda Endothelin-1 mediates hypoxia-induced inhibition of voltage-gated K+ channel expression in pulmonary arterial myocytes Am J Physiol Lung Cell Mol Physiol, February 1, 2008; 294(2): L309 - L318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Platoshyn, Y. Yu, E. A Ko, C. V. Remillard, and J. X.-J. Yuan Heterogeneity of hypoxia-mediated decrease in IK(V) and increase in [Ca2+]cyt in pulmonary artery smooth muscle cells Am J Physiol Lung Cell Mol Physiol, August 1, 2007; 293(2): L402 - L416. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Schach, M. Xu, O. Platoshyn, S. H. Keller, and J. X.-J. Yuan Thiol oxidation causes pulmonary vasodilation by activating K+ channels and inhibiting store-operated Ca2+ channels Am J Physiol Lung Cell Mol Physiol, March 1, 2007; 292(3): L685 - L698. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Wareing, X. Bai, F. Seghier, C. M. Turner, S. L. Greenwood, P. N. Baker, M. J. Taggart, and G. K. Fyfe Expression and function of potassium channels in the human placental vasculature Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2006; 291(2): R437 - R446. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Resnik, J. Herron, M. Keck, D. Sukovich, B. Linden, and D. N. Cornfield Chronic intrauterine pulmonary hypertension selectively modifies pulmonary artery smooth muscle cell gene expression Am J Physiol Lung Cell Mol Physiol, March 1, 2006; 290(3): L426 - L433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. T. Ward, T. P. Robertson, and P. I. Aaronson Capacitative calcium entry: a central role in hypoxic pulmonary vasoconstriction? Am J Physiol Lung Cell Mol Physiol, July 1, 2005; 289(1): L2 - L4. [Full Text] [PDF]
|
|
|
|
|
|
L. Weigand, J. Foxson, J. Wang, L. A. Shimoda, and J. T. Sylvester Inhibition of hypoxic pulmonary vasoconstriction by antagonists of store-operated Ca2+ and nonselective cation channels Am J Physiol Lung Cell Mol Physiol, July 1, 2005; 289(1): L5 - L13. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wang, L. Weigand, W. Wang, J. T. Sylvester, and L. A. Shimoda Chronic hypoxia inhibits Kv channel gene expression in rat distal pulmonary artery Am J Physiol Lung Cell Mol Physiol, June 1, 2005; 288(6): L1049 - L1058. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wang, L. A. Shimoda, L. Weigand, W. Wang, D. Sun, and J. T. Sylvester Acute hypoxia increases intracellular [Ca2+] in pulmonary arterial smooth muscle by enhancing capacitative Ca2+ entry Am J Physiol Lung Cell Mol Physiol, June 1, 2005; 288(6): L1059 - L1069. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Keck, E. Resnik, B. Linden, F. Anderson, D. J. Sukovich, J. Herron, and D. N. Cornfield Oxygen increases ductus arteriosus smooth muscle cytosolic calcium via release of calcium from inositol triphosphate-sensitive stores Am J Physiol Lung Cell Mol Physiol, May 1, 2005; 288(5): L917 - L923. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. C. Linden, E. R. Resnik, K. J. Hendrickson, J. M. Herron, T. J. O'Connor, and D. N. Cornfield Chronic intrauterine pulmonary hypertension compromises fetal pulmonary artery smooth muscle cell O2 sensing Am J Physiol Lung Cell Mol Physiol, December 1, 2003; 285(6): L1354 - L1361. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Cogo, G. Napolitano, M. C. Michoud, D. R. Barbon, M. Ward, and J. G. Martin Effects of hypoxia on rat airway smooth muscle cell proliferation J Appl Physiol, April 1, 2003; 94(4): 1403 - 1409. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Russell, N. J. Pelaez, C. S. Packer, M. E. Forster, and K. R. Olson Intracellular and extracellular calcium utilization during hypoxic vasoconstriction of cyclostome aortas Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2001; 281(5): R1506 - R1513. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Weissmann, F. Grimminger, A. Olschewski, and W. Seeger Hypoxic pulmonary vasoconstriction: a multifactorial response? Am J Physiol Lung Cell Mol Physiol, August 1, 2001; 281(2): L314 - L317. [Full Text] [PDF]
|
|
|
|
|
|
M. T. Rhodes, V. A. Porter, C. B. Saqueton, J. M. Herron, E. R. Resnik, and D. N. Cornfield Pulmonary vascular response to normoxia and KCa channel activity is developmentally regulated Am J Physiol Lung Cell Mol Physiol, June 1, 2001; 280(6): L1250 - L1257. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Liu, J. S. K. Sham, L. A. Shimoda, and J. T. Sylvester Hypoxic constriction of porcine distal pulmonary arteries: endothelium and endothelin dependence Am J Physiol Lung Cell Mol Physiol, May 1, 2001; 280(5): L856 - L865. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Shimoda, J. S. K. Sham, T. H. Shimoda, and J. T. Sylvester L-type Ca2+ channels, resting [Ca2+]i, and ET-1-induced responses in chronically hypoxic pulmonary myocytes Am J Physiol Lung Cell Mol Physiol, November 1, 2000; 279(5): L884 - L894. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. K. Sham, B. R. Crenshaw Jr., L.-H. Deng, L. A. Shimoda, and J. T. Sylvester Effects of hypoxia in porcine pulmonary arterial myocytes: roles of KV channel and endothelin-1 Am J Physiol Lung Cell Mol Physiol, August 1, 2000; 279(2): L262 - L272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. N. Cornfield, C. B. Saqueton, V. A. Porter, J. Herron, E. Resnik, I. Y. Haddad, and H. L. Reeve Voltage-gated K+-channel activity in ovine pulmonary vasculature is developmentally regulated Am J Physiol Lung Cell Mol Physiol, June 1, 2000; 278(6): L1297 - L1304. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Seiden, O. Platoshyn, A. E. Bakst, S. S. McDaniel, and J. X.-J. Yuan High K+-induced membrane depolarization attenuates endothelium-dependent pulmonary vasodilation Am J Physiol Lung Cell Mol Physiol, February 1, 2000; 278(2): L261 - L267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. L. Reeve, E. K. Weir, S. L. Archer, and D. N. Cornfield A maturational shift in pulmonary K+ channels, from Ca2+ sensitive to voltage dependent Am J Physiol Lung Cell Mol Physiol, December 1, 1998; 275(6): L1019 - L1025. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Sahai, C. Mei, T. A. Pattison, and R. L. Tannen Chronic hypoxia induces proliferation of cultured mesangial cells: role of calcium and protein kinase C Am J Physiol Renal Physiol, December 1, 1997; 273(6): F954 - F960. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Karaki, H. Ozaki, M. Hori, M. Mitsui-Saito, K.-I. Amano, K.-I. Harada, S. Miyamoto, H. Nakazawa, K.-J. Won, and K. Sato Calcium Movements, Distribution, and Functions in Smooth Muscle Pharmacol. Rev., June 1, 1997; 49(2): 157 - 230. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Dipp and A. M. Evans Cyclic ADP-Ribose Is the Primary Trigger for Hypoxic Pulmonary Vasoconstriction in the Rat Lung In Situ Circ. Res., July 6, 2001; 89(1): 77 - 83. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
