AJP - Lung Cellular and Molecular Physiology, Vol 265, Issue 4 360-L368, Copyright © 1993 by American Physiological Society

ARTICLES

Corticosteroids differentially regulate secretion of IL-6, IL-8, and G-CSF by a human bronchial epithelial cell line

S. J. Levine, P. Larivee, C. Logun, C. W. Angus and J. H. Shelhamer
Critical Care Medicine Department, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892.

Human airway epithelial cells play an active role in modulating airway inflammation by elaborating a variety of proinflammatory molecules, including cytokines. The purpose of this study was to define the role of corticosteroids in the regulation of cytokine gene transcription and secretion by human bronchial epithelial cells. In particular, we assessed whether dexamethasone was capable of inhibiting the tumor necrosis factor-alpha (TNF-alpha)-mediated secretion of interleukin-6 (IL-6), interleukin-8 (IL-8), and granulocyte colony-stimulating factor (G-CSF) by a human bronchial epithelial cell line (BEAS-2B). Stimulation with 20 ng/ml of TNF-alpha resulted in significant increases in secretion of immunoreactive IL-6, IL-8, and G-CSF that were maximal at 24 h. TNF-alpha-mediated IL-6, IL-8, and G-CSF secretion was concentration dependent and specific. In addition, stimulation with TNF-alpha resulted in significant increases in the quantity of IL-6, IL-8, and G-CSF mRNA as detected by reverse-transcription polymerase chain reaction. Dexamethasone preconditioning significantly inhibited both the secretion of immunoreactive IL-6 and the accumulation of IL-6 mRNA. Although dexamethasone appeared to reduce both the secretion of immunoreactive IL-8 and accumulation of IL-8 mRNA, the inhibitory effects did not reach statistical significance. Finally, dexamethasone did not inhibit either the secretion of immunoreactive G-CSF or the accumulation of G-CSF mRNA. In summary, our results suggest that corticosteroids have a differential effect on the regulation of cytokine secretion by human bronchial epithelial cells.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				G. Fingerle-Rowson, P. Koch, R. Bikoff, X. Lin, C. N. Metz, F. S. Dhabhar, A. Meinhardt, and R. Bucala Regulation of Macrophage Migration Inhibitory Factor Expression by Glucocorticoids in Vivo Am. J. Pathol., January 1, 2003; 162(1): 47 - 56. [Abstract] [Full Text] [PDF]

				S. Tavakoli, M. J. Cowan, T. Benfield, C. Logun, and J. H. Shelhamer Prostaglandin E2-induced interleukin-6 release by a human airway epithelial cell line Am J Physiol Lung Cell Mol Physiol, January 1, 2001; 280(1): L127 - L133. [Abstract] [Full Text] [PDF]

				N. Kotani, T. Kushikata, H. Hashimoto, F. Kimura, M. Muraoka, M. Yodono, M. Asai, and A. Matsuki Intrathecal Methylprednisolone for Intractable Postherpetic Neuralgia N. Engl. J. Med., November 23, 2000; 343(21): 1514 - 1519. [Abstract] [Full Text] [PDF]

				S. McKay, S. J. Hirst, M. B.-d. Haas, J. C. de Jongste, H. C. Hoogsteden, P. R. Saxena, and H. S. Sharma Tumor Necrosis Factor-alpha Enhances mRNA Expression and Secretion of Interleukin-6 in Cultured Human Airway Smooth Muscle Cells Am. J. Respir. Cell Mol. Biol., July 1, 2000; 23(1): 103 - 111. [Abstract] [Full Text]

				T. Suzuki, M. Yamaya, K. Sekizawa, N. Yamada, K. Nakayama, S. Ishizuka, M. Kamanaka, T. Morimoto, Y. Numazaki, and H. Sasaki Effects of dexamethasone on rhinovirus infection in cultured human tracheal epithelial cells Am J Physiol Lung Cell Mol Physiol, March 1, 2000; 278(3): L560 - L571. [Abstract] [Full Text] [PDF]

				J. Atsuta, J. Plitt, B. S. Bochner, and R. P. Schleimer Inhibition of VCAM-1 Expression in Human Bronchial Epithelial Cells by Glucocorticoids Am. J. Respir. Cell Mol. Biol., April 1, 1999; 20(4): 643 - 650. [Abstract] [Full Text]

				M. Fuhrmann, H.-U. Jahn, J. Seybold, C. Neurohr, P. J. Barnes, S. Hippenstiel, H. J. Kraemer, and N. Suttorp Identification and Function of Cyclic Nucleotide Phosphodiesterase Isoenzymes in Airway Epithelial Cells Am. J. Respir. Cell Mol. Biol., February 1, 1999; 20(2): 292 - 302. [Abstract] [Full Text]

				Z. Jiang, M. Kunimoto, and J. A. Patel Autocrine Regulation and Experimental Modulation of Interleukin-6 Expression by Human Pulmonary Epithelial Cells Infected with Respiratory Syncytial Virus J. Virol., March 1, 1998; 72(3): 2496 - 2499. [Abstract] [Full Text] [PDF]

				S. P. Sanders, E. S. Siekierski, J. D. Porter, S. M. Richards, and D. Proud Nitric Oxide Inhibits Rhinovirus-Induced Cytokine Production and Viral Replication in a Human Respiratory Epithelial Cell Line J. Virol., February 1, 1998; 72(2): 934 - 942. [Abstract] [Full Text] [PDF]

				C. Hierholzer, E. Kelly, K. Tsukada, E. Loeffert, S. Watkins, T. R. Billiar, and D. J. Tweardy Hemorrhagic shock induces G-CSF expression in bronchial epithelium Am J Physiol Lung Cell Mol Physiol, November 1, 1997; 273(5): L1058 - L1064. [Abstract] [Full Text] [PDF]

				L. M. Schwiebert, J. L. Mooney, S. Van Horn, A. Gupta, and R. P. Schleimer Identification of Novel Inducible Genes in Airway Epithelium Am. J. Respir. Cell Mol. Biol., July 1, 1997; 17(1): 106 - 113. [Abstract] [Full Text]