AJP - Lung Cellular and Molecular Physiology, Vol 266, Issue 1 46-L52, Copyright © 1994 by American Physiological Society

ARTICLES

Serotonin produces both hyperplasia and hypertrophy of bovine pulmonary artery smooth muscle cells in culture

S. L. Lee, W. W. Wang, J. J. Lanzillo and B. L. Fanburg
Department of Medicine, New England Medical Center, Boston, Massachusetts 02111.

Serotonin [5-hydroxytryptamine (5-HT)] has a dual effect on bovine pulmonary artery smooth muscle cells (SMC) in culture (S.-L. Lee, W. W. Wang, B. J. Moore, and B. L. Fanburg. Circ. Res. 68: 1362-1368, 1991.). Cellular internalization of 5-HT stimulates DNA synthesis and cellular proliferation, whereas the action of 5-HT on a cell surface receptor elevates adenosine 3',5'-cyclic monophosphate and inhibits proliferation. The present study shows that 5-HT causes proliferation of both pulmonary artery and aortic SMC but not of endothelial cells or fibroblasts. Furthermore, c-myc and alpha- and beta-actin gene expressions of pulmonary artery SMC were elevated after 2-h incubation with 5-HT, before stimulation of [3H]thymidine incorporation. Actinomycin D (0.05 micrograms/ml) but not cycloheximide (1 microgram/ml) inhibited the gene expression produced by 5-HT. Growth-arrested SMC progressed from a G0 quiescent state through a normal cell cycle when subjected to 5-HT, 5-HT plus 25 ng/ml insulin-like growth factor, or 5-HT plus 0.5 ng/ml fibroblast growth factor. Cell number increased by 20-40% at 40 h and 50-140% at 7 days. Protein content of cells treated with 5-HT was elevated by 20-40% at 7 days, whereas the rate of protein synthesis, measured by [35S]methionine incorporation, increased by 50-70% at 24 h. In the presence of 1 microM 5-HT, cells enlarged by 70 and 100-200% at 40 h and 7 days, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				S. Laudi, S. Trump, V. Schmitz, J. West, I. F. McMurtry, H. Mutlak, U. Christians, J. Weimann, U. Kaisers, and W. Steudel Serotonin transporter protein in pulmonary hypertensive rats treated with atorvastatin Am J Physiol Lung Cell Mol Physiol, September 1, 2007; 293(3): L630 - L638. [Abstract] [Full Text] [PDF]

				M. Sweeney, Y. Yu, O. Platoshyn, S. Zhang, S. S. McDaniel, and J. X.-J. Yuan Inhibition of endogenous TRP1 decreases capacitative Ca2+ entry and attenuates pulmonary artery smooth muscle cell proliferation Am J Physiol Lung Cell Mol Physiol, July 1, 2002; 283(1): L144 - L155. [Abstract] [Full Text] [PDF]

				V. J. Thannickal and B. L. Fanburg Reactive oxygen species in cell signaling Am J Physiol Lung Cell Mol Physiol, December 1, 2000; 279(6): L1005 - L1028. [Abstract] [Full Text] [PDF]

				R. B. Rothman, M. A. Ayestas, C. M. Dersch, and M. H. Baumann Aminorex, Fenfluramine, and Chlorphentermine Are Serotonin Transporter Substrates : Implications for Primary Pulmonary Hypertension Circulation, August 24, 1999; 100(8): 869 - 875. [Abstract] [Full Text] [PDF]

				C. Lu, F. J. Giordano, X. Bao, K. C. Morris, and A. Rothman Antisense fosB RNA Inhibits Thrombin-Induced Hypertrophy in Cultured Pulmonary Arterial Smooth Muscle Cells Circulation, August 11, 1998; 98(6): 596 - 603. [Abstract] [Full Text] [PDF]

				J. A. Florian and S. W. Watts Integration of Mitogen-Activated Protein Kinase Kinase Activation in Vascular 5-Hydroxytryptamine2A Receptor Signal Transduction J. Pharmacol. Exp. Ther., January 1, 1998; 284(1): 346 - 355. [Abstract] [Full Text]

				E. Nozik-Grayck, T. J. McMahon, Y.-C. T. Huang, C. S. Dieterle, J. S. Stamler, and C. A. Piantadosi Pulmonary vasoconstriction by serotonin is inhibited by S-nitrosoglutathione Am J Physiol Lung Cell Mol Physiol, May 1, 2002; 282(5): L1057 - L1065. [Abstract] [Full Text] [PDF]