AJP - Lung Cellular and Molecular Physiology, Vol 267, Issue 5 508-L517, Copyright © 1994 by American Physiological Society
Donor lung preservation: effect of cold preservation fluids on cultured pulmonary endothelial cells
S. M. Hall, H. Komai, J. Reader and S. G. Haworth
Developmental Vascular Biology and Pharmacology Unit, Institute of Child Health, London, United Kingdom.
Pulmonary arterial (PA) endothelial cell morphology changes during cold
preservation. In the present study, the efficacy of University of Wisconsin
solution (UW), UW solution without colloid (modified UW), Euro-Collins
(EC), Marshall's solution (MS), and medium 199 + 10% fetal calf serum
[culture medium (CM)] in maintaining and regaining the cytoskeleton of
cultured porcine PA endothelial cells kept at 4 degrees C and then rewarmed
was compared. Features studied were actin, microtubules, vinculin, and
talin, using immunofluorescence, sodium dodecyl sulfate-polyacrylamide gel
electrophoresis, and immunoblotting; permeability of the cell sheet; wound
healing; and phagocytic capacity. When cooled, microtubules depolymerized
in all fluids but EC and MS reduced depolymerization of actin. Permeability
decreased at 4 degrees C (P < 0.05), and wound healing and phagocytosis
ceased. When rewarmed after EC, UW, and CM preservation, wound healing and
phagocytosis started within 15 min and proceeded normally. Permeability
returned to normal but was excessive following UW preservation. Microtubule
repolymerization was fastest following UW preservation, and actin filament
repolymerization was fastest after EC preservation. Thus the type of
preservation fluid used influenced the rate of loss and recovery of
specific cytoskeletal components, with EC giving the fastest structural and
functional recovery.
