AJP - Lung Cellular and Molecular Physiology, Vol 267, Issue 5 564-L568, Copyright © 1994 by American Physiological Society
Interferon-gamma regulation of interleukin 6 in monocytic cells
D. E. Biondillo, S. A. Konicek and G. K. Iwamoto
Department of Medicine, Indiana University School of Medicine, Indianapolis 46202.
The regulation of interleukin 6 (IL-6) is dependent on many factors that
include numerous stimuli such as lipopolysaccharide (LPS), viruses, and
other cytokines. These studies demonstrate the ability of interferon-gamma
(IFN-gamma) to significantly enhance IL-6 mRNA and protein production in
LPS-stimulated monocytes and THP-1 cells. IL-6 protein production was
increased sevenfold in LPS-stimulated cells with the addition of IFN-gamma.
IL-6 mRNA production was increased in a dose-dependent fashion up to
15-fold in LPS-stimulated cells with the addition of IFN-gamma. The
enhanced production of IL-6 mRNA that occurs with the addition of IFN-gamma
to LPS-stimulated monocytic cells was due to increased transcription of
IL-6 mRNA. The ability of IFN-gamma to enhance IL-6 production may play an
important role in many inflammatory processes.
