AJP - Lung Cellular and Molecular Physiology, Vol 268, Issue 5 723-L728, Copyright © 1995 by American Physiological Society
Effects of cigarette smoke extract on bovine bronchial epithelial cell attachment and migration
D. E. Cantral, J. H. Sisson, T. Veys, S. I. Rennard and J. R. Spurzem
Department of Veterans Affairs Medical Center, Omaha 68105, USA.
The repair of injured epithelium involves a complex interaction between
epithelial cells and the underlying extracellular matrix. We studied the
effects of sublethal concentrations of cigarette smoke extract (CSE) and
two volatile components of cigarette smoke, acetaldehyde and acrolein, on
bovine bronchial epithelial cell (BBEC) attachment and migration in vitro.
After short-term exposure (2 and 6 h) to CSE, BBEC attachment to
fibronectin-coated dishes was decreased, and migration to fibronectin was
unchanged. After a longer period of exposure, 24 h, attachment was
increased and migration was unchanged. Exposure to a peptide containing the
amino acid sequence arginine-glycine-aspartic acid (RGD) reduced attachment
equally well for control and smoke-exposed cells. BBEC sheet migration was
reduced over 72 h after exposure to CSE. Acrolein reduced BBEC migration to
fibronectin but had no effect on attachment. Acetaldehyde had no effect on
either attachment or migration. We conclude that exposure to CSE has
important effects on bronchial epithelial cell migration and attachment,
and that these effects change over time.
