AJP - Lung Cellular and Molecular Physiology, Vol 270, Issue 5 729-L735, Copyright © 1996 by American Physiological Society

ARTICLES

Hemorrhage activates NF-kappa B in murine lung mononuclear cells in vivo

R. Shenkar, M. D. Schwartz, L. S. Terada, J. E. Repine, J. McCord and E. Abraham
Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado Health Sciences Center, Denver, USA.

Hemorrhage rapidly increases the expression of proinflammatory and immunoregulatory cytokines in the lungs. Binding elements for the nuclear transcriptional regulatory factors (NF)-kappa B and NF-IL6 (C/EBP beta) are present in the promoter regions of multiple cytokine genes, including those whose expression is increased after blood loss. In the present experiments, we found increased activation in vivo of NF-kappa B in lung mononuclear cells, but not in splenocytes, taken from mice 1 h after hemorrhage. In contrast, hemorrhage did not activate NF-IL6 in lung cells or splenocytes. Inhibition of xanthine oxidase by prior feeding of a tungsten-enriched diet prevented hemorrhage-induced activation in lung cells of NF-kappa B. Incubating splenocytes in vitro with xanthine oxidase activated NF-kappa B but not NF-IL6. Xanthine oxidase-induced activation of NF-kappa B was inhibited by manganese superoxide dismutase, but not by catalase. These results suggest that xanthine oxidase-mediated superoxide anion-dependent activation of NF-kappa B occurs in vivo and in vitro. This mechanism may contribute to increased lung cytokine responses after hemorrhage.

This article has been cited by other articles:

				X. Zhao, J. W. Zmijewski, E. Lorne, G. Liu, Y.-J. Park, Y. Tsuruta, and E. Abraham Activation of AMPK attenuates neutrophil proinflammatory activity and decreases the severity of acute lung injury Am J Physiol Lung Cell Mol Physiol, September 1, 2008; 295(3): L497 - L504. [Abstract] [Full Text] [PDF]

				E. Lorne, J. W. Zmijewski, X. Zhao, G. Liu, Y. Tsuruta, Y.-J. Park, H. Dupont, and E. Abraham Role of extracellular superoxide in neutrophil activation: interactions between xanthine oxidase and TLR4 induce proinflammatory cytokine production Am J Physiol Cell Physiol, April 1, 2008; 294(4): C985 - C993. [Abstract] [Full Text] [PDF]

				J. G. Kiang, R. M. Peckham, L. E. Duke, T. Shimizu, I. H. Chaudry, and G. C. Tsokos Androstenediol inhibits the trauma-hemorrhage-induced increase in caspase-3 by downregulating the inducible nitric oxide synthase pathway J Appl Physiol, March 1, 2007; 102(3): 933 - 941. [Abstract] [Full Text] [PDF]

				K. A. Barsness, J. Arcaroli, A. H. Harken, E. Abraham, A. Banerjee, L. Reznikov, and R. C. McIntyre Hemorrhage-induced acute lung injury is TLR-4 dependent Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2004; 287(3): R592 - R599. [Abstract] [Full Text] [PDF]

				R. P. Bowler, J. Arcaroli, E. Abraham, M. Patel, L.-Y. Chang, and J. D. Crapo Evidence for extracellular superoxide dismutase as a mediator of hemorrhage-induced lung injury Am J Physiol Lung Cell Mol Physiol, April 1, 2003; 284(4): L680 - L687. [Abstract] [Full Text] [PDF]

				J. G. Kupfner, J. J. Arcaroli, H.-K. Yum, S. G. Nadler, K.-Y. Yang, and E. Abraham Role of NF-{kappa}B in Endotoxemia-Induced Alterations of Lung Neutrophil Apoptosis J. Immunol., December 15, 2001; 167(12): 7044 - 7051. [Abstract] [Full Text] [PDF]

				H.-K. Yum, J. Arcaroli, J. Kupfner, R. Shenkar, J. M. Penninger, T. Sasaki, K.-Y. Yang, J. S. Park, and E. Abraham Involvement of Phosphoinositide 3-Kinases in Neutrophil Activation and the Development of Acute Lung Injury J. Immunol., December 1, 2001; 167(11): 6601 - 6608. [Abstract] [Full Text] [PDF]

				L. J. Landesberg, R. Ramalingam, K. Lee, T. K. Rosengart, and R. G. Crystal Upregulation of transcription factors in lung in the early phase of postpneumonectomy lung growth Am J Physiol Lung Cell Mol Physiol, November 1, 2001; 281(5): L1138 - L1149. [Abstract] [Full Text] [PDF]

				J.-F. Pittet, L. N. Lu, D. G. Morris, K. Modelska, W. J. Welch, H. V. Carey, J. Roux, and M. A. Matthay Reactive Nitrogen Species Inhibit Alveolar Epithelial Fluid Transport After Hemorrhagic Shock in Rats J. Immunol., May 15, 2001; 166(10): 6301 - 6310. [Abstract] [Full Text] [PDF]

				E. Abraham, J. Arcaroli, and R. Shenkar Activation of Extracellular Signal-Regulated Kinases, NF-{{kappa}}B, and Cyclic Adenosine 5'-Monophosphate Response Element-Binding Protein in Lung Neutrophils Occurs by Differing Mechanisms After Hemorrhage or Endotoxemia J. Immunol., January 1, 2001; 166(1): 522 - 530. [Abstract] [Full Text] [PDF]

				E. Abraham Coagulation Abnormalities in Acute Lung Injury and Sepsis Am. J. Respir. Cell Mol. Biol., April 1, 2000; 22(4): 401 - 404. [Full Text]

				J. Fan, A. Kapus, Y. H. Li, S. Rizoli, J. C. Marshall, and O. D. Rotstein Priming for Enhanced Alveolar Fibrin Deposition after Hemorrhagic Shock . Role of Tumor Necrosis Factor Am. J. Respir. Cell Mol. Biol., April 1, 2000; 22(4): 412 - 421. [Abstract] [Full Text]

				R. Shenkar and E. Abraham Mechanisms of Lung Neutrophil Activation After Hemorrhage or Endotoxemia: Roles of Reactive Oxygen Intermediates, NF-{kappa}B, and Cyclic AMP Response Element Binding Protein J. Immunol., July 15, 1999; 163(2): 954 - 962. [Abstract] [Full Text] [PDF]

				J. Fan, J. C. Marshall, M. Jimenez, P. N. Shek, J. Zagorski, and O. D. Rotstein Hemorrhagic Shock Primes for Increased Expression of Cytokine-Induced Neutrophil Chemoattractant in the Lung: Role in Pulmonary Inflammation Following Lipopolysaccharide J. Immunol., July 1, 1998; 161(1): 440 - 447. [Abstract] [Full Text] [PDF]