AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 275: L717-L728, 1998;
1040-0605/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ceneviva, G. D.
Right arrow Articles by Pitt, B. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ceneviva, G. D.
Right arrow Articles by Pitt, B. R.
Vol. 275, Issue 4, L717-L728, October 1998

Nitric oxide inhibits lipopolysaccharide-induced apoptosis in pulmonary artery endothelial cells

Gary D. Ceneviva1, Edith Tzeng2, Dale G. Hoyt3, Emily Yee4, Alicia Gallagher3, John F. Engelhardt5, Young-Myeong Kim2,3, Timothy R. Billiar2, Simon A. Watkins6, and Bruce R. Pitt1,3

3 Department of Pharmacology; 1 Division of Pediatric Critical Care Medicine, Department of Anesthesiology; 2 Department of Surgery; 4 Division of Pulmonary, Allergy, and Critical Care Medicine; and 6 Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261; and 5 Department of Anatomy and Cell Biology, University of Iowa Medical Center, Iowa City, Iowa 52242

Our group recently reported that cultured sheep pulmonary artery endothelial cells (SPAECs) became resistant to lipopolysaccharide (LPS)-induced apoptosis several days after constitutive synthesis of nitric oxide (NO) after adenoviral (Ad) transfer of inducible NO synthase (iNOS) or exposure to the NO donor S-nitroso-N-acetylpenicillamine (SNAP) (E. Tzeng, Y.-M. Kim, B. R. Pitt, A. Lizonova, I. Kovesdi, and T. R. Billiar. Surgery 122: 255-263, 1997). In the present study, we confirmed this observation by establishing stable transfectants after retroviral gene transfer [replication-deficient retrovirus (DFG)] of human iNOS (DFG-iNOS) SPAECs and then used all three approaches (Ad, DFG, and SNAP) to determine underlying mechanisms of this phenomenon. Continuous endogenous production of NO in itself did not cause apoptosis as assessed by phase-contrast microscopy, nuclear morphology, and internucleosomal DNA fragmentation. Prolonged (72-96 h) synthesis of NO, however, after DFG- or replication-deficient adenovirus (Ad.CMV)-iNOS or SNAP (100 µM, 96 h) inhibited LPS-induced apoptosis. The kinetics of such protection suggested that NO may be inducing other gene products. Ad-mediated transfer of manganese superoxide dismutase (MnSOD) decreased the sensitivity of wild-type SPAECs to LPS-induced apoptosis. MnSOD, however, was not induced in an NG-monomethyl-L-arginine (L-NMMA)-sensitive time-dependent fashion after Ad.CMV-iNOS. Other inducible genes that may be affected by NO and that may protect against potential oxidant-mediated LPS-induced apoptosis including 70-kDa heat shock protein, heme oxygenase-1, metallothionein, and Bcl-2 also were not elevated in an L-NMMA-sensitive, time-dependent fashion. Although the candidate gene product underlying NO-induced protection remains unclear, we did note that prolonged synthesis of NO inhibited LPS-induced activation of an interleukin-1beta -converting enzyme-like cysteine protease (cysteine protease protein-32-like) in a dithiothreitol-sensitive fashion, suggesting that S-nitrosylation of an important downstream target of convergence of apoptotic signals may contribute to the sensitivity of SPAECs to LPS.

cysteine protease protein-32-like protease; manganese superoxide dismutase; mitochondria; heme oxygenase; Bcl-2


This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
C. Yang, B. R. Mwaikambo, T. Zhu, C. Gagnon, J. Lafleur, S. Seshadri, P. Lachapelle, J.-C. Lavoie, S. Chemtob, and P. Hardy
Lymphocytic microparticles inhibit angiogenesis by stimulating oxidative stress and negatively regulating VEGF-induced pathways
Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2008; 294(2): R467 - R476.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
A. Lu, M. Frink, M. A. Choudhry, W. J. Hubbard, L. W. Rue III, K. I. Bland, and I. H. Chaudry
Mitochondria play an important role in 17beta-estradiol attenuation of H2O2-induced rat endothelial cell apoptosis
Am J Physiol Endocrinol Metab, February 1, 2007; 292(2): E585 - E593.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
F. D'Agnillo
Redox active hemoglobin enhances lipopolysaccharide-induced injury to cultured bovine endothelial cells
Am J Physiol Heart Circ Physiol, October 1, 2004; 287(4): H1875 - H1882.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. C. Rudkowski, E. Barreiro, R. Harfouche, P. Goldberg, O. Kishta, P. D'Orleans-Juste, J. Labonte, O. Lesur, and S. N. A. Hussain
Roles of iNOS and nNOS in sepsis-induced pulmonary apoptosis
Am J Physiol Lung Cell Mol Physiol, April 1, 2004; 286(4): L793 - L800.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
K. Hemmrich, C. V. Suschek, G. Lerzynski, and V. Kolb-Bachofen
iNOS activity is essential for endothelial stress gene expression protecting against oxidative damage
J Appl Physiol, November 1, 2003; 95(5): 1937 - 1946.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. D. Bannerman and S. E. Goldblum
Mechanisms of bacterial lipopolysaccharide-induced endothelial apoptosis
Am J Physiol Lung Cell Mol Physiol, June 1, 2003; 284(6): L899 - L914.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
M. AMON, M. D. MENGER, and B. VOLLMAR
Heme oxygenase and nitric oxide synthase mediate cooling-associated protection against TNF-{alpha}-induced microcirculatory dysfunction and apoptotic cell death
FASEB J, February 1, 2003; 17(2): 175 - 185.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. Hull, G. McLean, F. Wong, P. J. Duriez, and A. Karsan
Lipopolysaccharide Signals an Endothelial Apoptosis Pathway Through TNF Receptor-Associated Factor 6-Mediated Activation of c-Jun NH2-Terminal Kinase
J. Immunol., September 1, 2002; 169(5): 2611 - 2618.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
M. Afrouzian, V. Ramassar, J. Urmson, L.-F. Zhu, and P. F. Halloran
Transcription Factor IRF-1 in Kidney Transplants Mediates Resistance to Graft Necrosis during Rejection
J. Am. Soc. Nephrol., May 1, 2002; 13(5): 1199 - 1209.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
B. R. Pitt and C. M. St. Croix
Complex Regulation of iNOS in Lung
Am. J. Respir. Cell Mol. Biol., January 1, 2002; 26(1): 6 - 9.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. Jaiswal, N. F. LaRusso, and G. J. Gores
Nitric oxide in gastrointestinal epithelial cell carcinogenesis: linking inflammation to oncogenesis
Am J Physiol Gastrointest Liver Physiol, September 1, 2001; 281(3): G626 - G634.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
J. He, Y. Xiao, and L. Zhang
Cocaine-Mediated Apoptosis in Bovine Coronary Artery Endothelial Cells: Role of Nitric Oxide
J. Pharmacol. Exp. Ther., July 1, 2001; 298(1): 180 - 187.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Z.-L. Tang, K. Wasserloos, C. M. St. Croix, and B. R. Pitt
Role of zinc in pulmonary endothelial cell response to oxidative stress
Am J Physiol Lung Cell Mol Physiol, July 1, 2001; 281(1): L243 - L249.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
P. F. Halloran, M. Afrouzian, V. Ramassar, J. Urmson, L.-F. Zhu, L. M. H. Helms, K. Solez, and N. M. Kneteman
Interferon-{{gamma}} Acts Directly on Rejecting Renal Allografts to Prevent Graft Necrosis
Am. J. Pathol., January 1, 2001; 158(1): 215 - 226.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. P. Merker, B. R. Pitt, A. M. Choi, P. M. Hassoun, C. A. Dawson, and A. B. Fisher
Lung redox homeostasis: emerging concepts
Am J Physiol Lung Cell Mol Physiol, September 1, 2000; 279(3): L413 - L417.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y.-M. Kim, H.-T. Chung, R. L. Simmons, and T. R. Billiar
Cellular Non-heme Iron Content Is a Determinant of Nitric Oxide-mediated Apoptosis, Necrosis, and Caspase Inhibition
J. Biol. Chem., April 6, 2000; 275(15): 10954 - 10961.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
I. Petrache, L. E. Otterbein, J. Alam, G. W. Wiegand, and A. M. K. Choi
Heme oxygenase-1 inhibits TNF-alpha -induced apoptosis in cultured fibroblasts
Am J Physiol Lung Cell Mol Physiol, February 1, 2000; 278(2): L312 - L319.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
Y. Xiao, J. He, R. D. Gilbert, and L. Zhang
Cocaine Induces Apoptosis in Fetal Myocardial Cells through a Mitochondria-Dependent Pathway
J. Pharmacol. Exp. Ther., January 1, 2000; 292(1): 8 - 14.
[Abstract] [Full Text]

Home page
 ScienceHome page
J. B. Mannick, A. Hausladen, L. Liu, D. T. Hess, M. Zeng, Q. X. Miao, L. S. Kane, A. J. Gow, and J. S. Stamler
Fas-Induced Caspase Denitrosylation
Science, April 23, 1999; 284(5414): 651 - 654.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
Y.-G. Kwon, J.-K. Min, K.-M. Kim, D.-J. Lee, T. R. Billiar, and Y.-M. Kim
Sphingosine 1-Phosphate Protects Human Umbilical Vein Endothelial Cells from Serum-deprived Apoptosis by Nitric Oxide Production
J. Biol. Chem., March 30, 2001; 276(14): 10627 - 10633.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online