| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of 1 Biochemistry
and 3 Obstetrics-Gynecology and
4 The Cecil H. and Ida Green
Center for Reproductive Biology Sciences,
Surfactant protein (SP) A gene transcription is developmentally regulated and stimulated by hormones and factors that increase intracellular cAMP. The baboon (b) genome contains two highly similar SP-A genes, bSP-A1 and bSP-A2. With the use of a ribonuclease protection assay with gene-specific probes, the two bSP-A genes were found to be differentially regulated during baboon fetal lung development in that expression of the bSP-A2 gene appeared to be induced to a high level at a later time in gestation than that of the bSP-A1 gene. Both the bSP-A1 and bSP-A2 genes were found to be highly responsive to the inductive effects of cAMP in baboon fetal lung explants in culture. By DNase I footprinting and electrophoretic mobility shift assays with bacterially expressed thyroid transcription factor-1 (TTF-1) and type II cell nuclear extracts, three TTF-1 binding elements were identified within the 255-bp region flanking the 5'-end of each bSP-A gene; however, these differed in position and spacing for the two bSP-A genes. To functionally define the genomic regions that are required for cAMP regulation of bSP-A gene expression in type II cells, fusion genes composed of various amounts of 5'-flanking DNA from the bSP-A1 and bSP-A2 genes linked to the human growth hormone structural gene as a reporter were transfected into type II cells in primary culture. We found that 255 bp of 5'-flanking DNA, which contain three TTF-1 binding elements, from bSP-A1 and bSP-A2 genes were sufficient to mediate high basal and cAMP-inducible expression in type II cells. We also observed that there were no obvious differences in the magnitude of the responses of these fusion genes to cAMP treatment.
surfactant protein A; deoxyribonucleic acid; fetal lung; thyroid transcription factor-1; ribonuclease protection assay
This article has been cited by other articles:
|
|
 |
|
  D. Liu, M. Yi, M. Smith, and C. R. Mendelson TTF-1 response element is critical for temporal and spatial regulation and necessary for hormonal regulation of human surfactant protein-A2 promoter activity Am J Physiol Lung Cell Mol Physiol, August 1, 2008; 295(2): L264 - L271. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Alcorn, K. N. Islam, P. P. Young, and C. R. Mendelson Glucocorticoid inhibition of SP-A gene expression in lung type II cells is mediated via the TTF-1-binding element Am J Physiol Lung Cell Mol Physiol, April 1, 2004; 286(4): L767 - L776. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. DeMartini, J. V. Bishop, T. E. Allen, F. A. Jassim, J. M. Sharp, M. de las Heras, D. R. Voelker, and J. O. Carlson Jaagsiekte Sheep Retrovirus Proviral Clone JSRVJS7, Derived from the JS7 Lung Tumor Cell Line, Induces Ovine Pulmonary Carcinoma and Is Integrated into the Surfactant Protein A Gene J. Virol., May 1, 2001; 75(9): 4239 - 4246. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Yi, G.-X. Tong, B. Murry, and C. R. Mendelson Role of CBP/p300 and SRC-1 in Transcriptional Regulation of the Pulmonary Surfactant Protein-A (SP-A) Gene by Thyroid Transcription Factor-1 (TTF-1) J. Biol. Chem., January 18, 2002; 277(4): 2997 - 3005. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
