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Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55455
The Na+
pump, Na+-K+-ATPase, along with the
Na+ channel is essential for the
removal of alveolar solute and fluid perinatally. Because
Na+-pump mRNA and activity
increase before birth and maternal glucocorticoids (GCs) influence
Na+-K+-ATPase
mRNA expression in fetal rat lung, we hypothesized that GCs increased
Na+-K+-ATPase
gene expression in a fetal lung epithelial cell line. After 24 h of
exposure, dexamethasone increased the steady-state levels of
Na+-K+-ATPase
1 and
1 mRNA in a fetal rat lung
epithelial cell line in a dose-dependent fashion
(10
7 to
105 M). The maximal
increase in mRNA levels was 3.8-fold for
1 and 2.8-fold for
1. The increase in mRNA was
detected as early as 6 h for the
1-subunit and 18 h for the
1-subunit, and both peaked at
24 h. This gene upregulation was not due to increased mRNA stability
based on mRNA half-life determination after actinomycin D inhibition.
Transfection experiments with 1
and 1 promoter-reporter constructs demonstrated 3.2 ± 0.5- and 2.6 ± 0.4-fold
increases, respectively, in promoter activity, consistent with
transcriptional activation of the promoter-reporter construct. These
findings, increased promoter activity with no change in stability,
indicate that GCs increased
Na+-K+-ATPase
transcription in a fetal lung epithelial cell line.
sodium-potassium-adenosine 5'-triphosphatase; promoter
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