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Am J Physiol Lung Cell Mol Physiol 277: L218-L224, 1999;
1040-0605/99 $5.00
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Vol. 277, Issue 1, L218-L224, July 1999

Differential expression of platelet-derived growth factor-alpha receptor by Thy-1minus and Thy-1+ lung fibroblasts

James S. Hagood1, Patrick J. Miller2, Joseph A. Lasky3, Albert Tousson4, Benliu Guo1, Gerald M. Fuller4, and J. Clarke McIntosh5

Departments of 1 Pediatrics, 2 Microbiology, and 4 Cell Biology, University of Alabama at Birmingham, Birmingham, Alabama 35294; 3 Section of Pulmonary Diseases and Critical Care Medicine, Department of Medicine, Tulane University, New Orleans, Louisiana, 70112; and 5 The Ruth and Billy Graham Children's Medical Center, Asheville, North Carolina 28801

Fibroblasts are heterogeneous with respect to surface markers, morphology, and participation in fibrotic responses. This study was undertaken to determine whether Thy-1- and Thy-1+ rat lung fibroblasts, which have distinct and relevant phenotypes, differ in their proliferative responses to platelet-derived growth factor (PDGF) isoforms. Homogeneous populations of Thy-1- and Thy-1+ fibroblasts were found to proliferate equally in the presence of PDGF-BB, but PDGF-AA-mediated proliferation occurred only in Thy-1- cells. This differential activity correlated with significantly higher expression of PDGF-alpha receptor in Thy-1- fibroblasts as shown by immunoblotting, immunofluorescence, and Northern blotting. There was a rapid increase in c-myc mRNA in Thy-1- but not in Thy-1+ fibroblasts on stimulation with PDGF-AA and PDGF-BB. The PDGF-alpha receptor, which mediates signaling by all PDGF isoforms, has been implicated in numerous clinical and experimental forms of fibrosis and regulates lung morphogenesis. Differential expression of the PDGF-alpha receptor supports distinct roles for Thy-1- and Thy-1+ fibroblast populations in developmental and fibrotic processes in the lung.

cell surface molecules; rodent; proliferation


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