| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of 1 Anesthesiology, 2 Pathology, and 3 Anatomy and Cellular Biology, State University of New York-Health Science Center, Syracuse, New York 13210
The effect of hyperoxia on nitric oxide (NO) production in intact animals is unknown. We described the effects of hyperoxia on inducible nitric oxide synthase (iNOS) expression and NO production in the lungs of rats exposed to high concentrations of oxygen. Animals were placed in sealed Plexiglas chambers and were exposed to either 85% oxygen (hyperoxic group) or 21% oxygen (negative control group). Animals were anesthetized after 24 and 72 h of exposure and were ventilated via a tracheotomy. We measured NO production in exhaled air (ENO) by chemiluminescence. The lungs were then harvested and processed for detection of iNOS by immunohistochemistry and Western blotting analysis. The same experiments were repeated in animals exposed to hyperoxia for 72 h after they were infused with L-arginine. We used rats that were injected intraperitoneally with Escherichia coli lipopolysaccharide to induce septic shock as a positive control group. Hyperoxia and septic shock induced expression of iNOS in the lung. However, ENO was elevated only in septic shock rats but was normal in the hyperoxic group. Exogenous infusion of L-arginine after hyperoxia did not increase ENO. To exclude the possibility that in the hyperoxic group NO was scavenged by oxygen radicals to form peroxynitrite, lungs were studied by immunohistochemistry for the detection of nitrotyrosine. Nitrotyrosine was found in septic shock animals but not in the hyperoxic group, further suggesting that NO is not synthesized in rats exposed to hyperoxia. We conclude that hyperoxia induces iNOS expression in the lung without an increase in NO concentration in the exhaled air.
hyperoxia; septic shock
This article has been cited by other articles:
|
|
 |
|
  I. T. Demchenko, K. E. Welty-Wolf, B. W. Allen, and C. A. Piantadosi Similar but not the same: normobaric and hyperbaric pulmonary oxygen toxicity, the role of nitric oxide Am J Physiol Lung Cell Mol Physiol, July 1, 2007; 293(1): L229 - L238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Hampl, J. Bibova, A. Banasova, J. Uhlik, D. Mikova, O. Hnilickova, V. Lachmanova, and J. Herget Pulmonary vascular iNOS induction participates in the onset of chronic hypoxic pulmonary hypertension Am J Physiol Lung Cell Mol Physiol, January 1, 2006; 290(1): L11 - L20. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Belik, R. P. Jankov, J. Pan, M. Yi, I. Chaudhry, and A. K. Tanswell Chronic O2 exposure in the newborn rat results in decreased pulmonary arterial nitric oxide release and altered smooth muscle response to isoprostane J Appl Physiol, February 1, 2004; 96(2): 725 - 730. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Tahepold, A. Ruusalepp, G. Li, J. Vaage, J. Starkopf, and G. Valen Cardioprotection by breathing hyperoxic gas--relation to oxygen concentration and exposure time in rats and mice Eur. J. Cardiothorac. Surg., June 1, 2002; 21(6): 987 - 994. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Hayashi, Y. Sawa, N. Fukuyama, H. Nakazawa, and H. Matsuda Inducible nitric oxide production is an adaptation to cardiopulmonary bypass-induced inflammatory response Ann. Thorac. Surg., July 1, 2001; 72(1): 149 - 155. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Pepperl, M. Dorger, F. Ringel, C. Kupatt, and F. Krombach Hyperoxia upregulates the NO pathway in alveolar macrophages in vitro: role of AP-1 and NF-{kappa}B Am J Physiol Lung Cell Mol Physiol, May 1, 2001; 280(5): L905 - L913. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
