Vol. 277, Issue 5, L1051-L1056, November 1999
Hyperthermia enhances cytomegalovirus regulation of HIV-1 and
TNF-
gene expression
Gary K.
Iwamoto1,2,
Audrey M.
Ainsworth1, and
Pope L.
Moseley2
1 Medical Research Service,
Veterans Affairs Medical Center, Albuquerque 87108; and
2 Department of Medicine,
University of New Mexico School of Medicine, Albuquerque, New Mexico
87131
The
immediate-early (IE) genes of human cytomegalovirus
(CMV) can be expressed in monocytic cells and
are known to regulate viral and cellular genes. Reactivation of human
immunodeficiency virus (HIV-1) may be stimulated by a variety of
factors including other viruses and inflammatory cytokines. These
studies examine the role of hyperthermia and CMV in the regulation of
HIV-1 and tumor necrosis factor (TNF)-
. THP-1 cells were transfected
with the CMV IE genes. HIV-1 and TNF- transcription were
assessed with chloramphenicol acetyltransferase promoter constructs.
Hyperthermia sufficient to stimulate production of heat shock proteins
was used to stimulate the cells. Hyperthermia significantly enhances the effect of CMV IE gene products on the expression of
HIV-1 and TNF-. The increases in HIV-1 transcription appear to be in part due to increases in TNF-. Heat shock proteins induced by hyperthermia may play an important role in the viral regulation of
monocytic function by CMV.
THP-1 cells; endotoxin; tumor necrosis factor-; heat shock
proteins; human immunodeficiency virus
