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Am J Physiol Lung Cell Mol Physiol 277: L996-L1002, 1999;
1040-0605/99 $5.00
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Vol. 277, Issue 5, L996-L1002, November 1999

Expression of C-type natriuretic peptide during development of rat lung

Kuniaki Nakanishi1, Fumiko Tajima2, Hiroshi Itoh3, Yasuko Nakata2, Norio Hama3, Osamu Nakagawa3, Kazuwa Nakao3, Toshiaki Kawai4, Chikao Torikata4, Tatsuko Suga5, Kunio Takishima5, Takashi Aurues1, and Tomosumi Ikeda1

1 Division of Environmental Medicine, National Defense Medical College Research Institute, and Departments of 4 Pathology and 5 Biochemistry, National Defense Medical College, Tokorozawa 359-8513; 2 Biochemical Division, Aeromedical Laboratory, Japan Air Self-Defense Force, Tachikawa 190-0003; and 3 Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Kyoto 606-8507, Japan

C-type natriuretic peptide (CNP), recently found to be secreted from vascular endothelial cells, is now viewed as a novel endothelium-derived relaxing peptide. However, the distribution and expression of CNP during cardiopulmonary development is unclear. To follow changes in the expression of CNP during lung development, we examined rat embryos and neonates using Northern blot analysis and in situ hybridization for CNP mRNA and radioimmunoassay and immunohistochemistry for CNP protein. A substantial expression of CNP mRNA was first detected on postnatal day 2, and it thereafter remained fairly steady. The level of CNP protein also increased rapidly after postnatal day 1, reaching a settled level on postnatal day 4. CNP protein and mRNA were detected in the endothelium and smooth muscle cells of blood vessels and in bronchial airway and alveolar epithelia. Immunoreactivity for CNP protein in the endothelium of blood vessels increased to an intense level after the saccular stage. These results suggest that the changes in CNP levels may be related to the occurrence of pulmonary vasodilation after birth.

cardiopulmonary system


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