| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Research Service, Malcom Randall Department of Veterans Affairs Medical Center, and Department of Medicine, University of Florida, Gainesville, Florida 32608-1197
In this study, we
investigated the possible interaction between the cationic amino acid
transporter (CAT)-1 arginine transporter and ankyrin or
fodrin. Because ankyrin and fodrin are substrates for
calpain and because hypoxia increases calpain expression and activity
in pulmonary artery endothelial cells (PAEC), we also studied the
effect of hypoxia on ankyrin, fodrin, and CAT-1 contents in PAEC.
Exposure to long-term hypoxia (24 h) inhibited L-arginine uptake by PAEC, and this inhibition was prevented by calpain inhibitor 1. The effects of hypoxia and calpain inhibitor 1 were not associated with changes in CAT-1 transporter content in PAEC plasma membranes. However, hypoxia stimulated the hydrolysis of ankyrin and fodrin in
PAEC, and this could be prevented by calpain inhibitor 1. Incubation of
solubilized plasma membrane proteins with anti-fodrin antibodies resulted in a 70% depletion of CAT-1 immunoreactivity and in a 60%
decrease in L-arginine transport activity in reconstituted proteoliposomes (3,291 ± 117 vs. 8,101 ± 481 pmol · mg
protein
1 · 3 min1 in
control). Incubation with anti-ankyrin antibodies had
no effect on CAT-1 content or L-arginine transport in
reconstituted proteoliposomes. These results demonstrate that CAT-1
arginine transporters in PAEC are associated with fodrin, but not with
ankyrin, and that long-term hypoxia decreases L-arginine
transport by a calpain-mediated mechanism that may involve fodrin proteolysis.
ankyrin; calpain; hypoxia; pulmonary artery endothelial cells; cationic amino acid transporter-1
This article has been cited by other articles:
|
|
 |
|
  L. Ostergaard, E. Stankevicius, M. R. Andersen, Y. Eskildsen-Helmond, T. Ledet, M. J. Mulvany, and U. Simonsen Diminished NO release in chronic hypoxic human endothelial cells Am J Physiol Heart Circ Physiol, November 1, 2007; 293(5): H2894 - H2903. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Su, D. Kondrikov, and E. R. Block beta-Actin: A Regulator of NOS-3 Sci. Signal., September 18, 2007; 2007(404): pe52 - pe52. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. McCord, P. Ayuk, M. McMahon, R. C.A. Boyd, I. Sargent, and C. Redman System y+ Arginine Transport and NO Production in Peripheral Blood Mononuclear Cells in Pregnancy and Preeclampsia Hypertension, January 1, 2006; 47(1): 109 - 115. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Reboul, S. Tanguy, A. Gibault, M. Dauzat, and P. Obert Chronic hypoxia exposure depresses aortic endothelium-dependent vasorelaxation in both sedentary and trained rats: involvement of L-arginine J Appl Physiol, September 1, 2005; 99(3): 1029 - 1035. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. I. Closs, A. Simon, N. Vekony, and A. Rotmann Plasma Membrane Transporters for Arginine J. Nutr., October 1, 2004; 134(10): 2752S - 2759S. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Parnell, J. P. F. Chin-Dusting, J. Starr, and D. M. Kaye In vivo and in vitro evidence for ACh-stimulated L-arginine uptake Am J Physiol Heart Circ Physiol, July 1, 2004; 287(1): H395 - H400. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. R. Morris, S. M. Morris Jr., W. Hagar, J. van Warmerdam, S. Claster, D. Kepka-Lenhart, L. Machado, F. A. Kuypers, and E. P. Vichinsky Arginine Therapy: A New Treatment for Pulmonary Hypertension in Sickle Cell Disease? Am. J. Respir. Crit. Care Med., July 1, 2003; 168(1): 63 - 69. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Su, S. Edwards-Bennett, M. R. Bubb, and E. R. Block Regulation of endothelial nitric oxide synthase by the actin cytoskeleton Am J Physiol Cell Physiol, June 1, 2003; 284(6): C1542 - C1549. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. E. Mann, D. L. Yudilevich, and L. Sobrevia Regulation of Amino Acid and Glucose Transporters in Endothelial and Smooth Muscle Cells Physiol Rev, January 1, 2003; 83(1): 183 - 252. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kizhatil and L. M. Albritton System y+ localizes to different membrane subdomains in the basolateral plasma membrane of epithelial cells Am J Physiol Cell Physiol, December 1, 2002; 283(6): C1784 - C1794. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. D. Nelin, H. E. Nash, and L. G. Chicoine Cytokine treatment increases arginine metabolism and uptake in bovine pulmonary arterial endothelial cells Am J Physiol Lung Cell Mol Physiol, November 1, 2001; 281(5): L1232 - L1239. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. I. Zharikov, A. A. Sigova, S. Chen, M. R. Bubb, and E. R. Block Cytoskeletal regulation of the L-arginine/NO pathway in pulmonary artery endothelial cells Am J Physiol Lung Cell Mol Physiol, March 1, 2001; 280(3): L465 - L473. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
