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Am J Physiol Lung Cell Mol Physiol 278: L177-L184, 2000;
1040-0605/00 $5.00
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Vol. 278, Issue 1, L177-L184, January 2000

Early expression of beta - and gamma -subunits of epithelial sodium channel during human airway development

Dominique Gaillard1, Jocelyne Hinnrasky1, Sylvie Coscoy2, Paul Hofman3, Michael A. Matthay4, Edith Puchelle1, and Pascal Barbry2,4

1 Institut National de la Santé et de la Recherche Médicale Unité 514, Institut Fédératif de Recherches 53, Centre Hospitalier Universitaire Maison Blanche, 51092 Reims Cedex; 2 Institut de Pharmacologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique Unité Propre de Recherche 411, 06560 Sophia Antipolis; 3 Institut National de la Santé et de la Recherche Médicale Unité 364, Tour Pasteur Faculté de Médecine, 06107 Nice Cedex 02, France; and 4 Cardiovascular Research Institute, University of California, San Francisco, California 94143-0130

The amiloride-sensitive epithelial Na+ channel (ENaC) is an apical membrane protein complex involved in active Na+ absorption and in control of fluid composition in airways. There are no data reporting the distribution of its pore-forming alpha -, beta -, and gamma -subunits in the developing human lung. With use of two different rabbit polyclonal antisera raised against beta - and gamma -ENaC, immunohistochemical localization of the channel was performed in fetal (10-35 wk) and in adult human airways. Both subunits were detected after 17 wk of gestation on the apical domain of bronchial ciliated cells, in glandular ducts, and in bronchiolar ciliated and Clara cells. After 30 wk, the distribution of beta - and gamma -subunits was similar in fetal and adult airways. In large airways, the two subunits were detected in ciliated cells, in cells lining glandular ducts, and in the serous gland cells. In the distal bronchioles, beta - and gamma -subunits were identified in ciliated and Clara cells. Ultrastructural immunogold labeling confirmed the identification of beta - and gamma -ENaC proteins in submucosal serous cells and bronchiolar Clara cells. Early expression of ENaC proteins in human fetal airways suggests that Na+ absorption might begin significantly before birth, even if secretion is still dominant.

amiloride; human fetal development; airway epithelium; Clara cell; glandular cell


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