AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 278: L68-L74, 2000;
1040-0605/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (4)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rider, E. D.
Right arrow Articles by Jobe, A. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rider, E. D.
Right arrow Articles by Jobe, A. H.
Vol. 278, Issue 1, L68-L74, January 2000

Lysosomes from rabbit type II cells catabolize surfactant lipids

Evelyn D. Rider1, Machiko Ikegami2, Kent E. Pinkerton3, Janice L. Peake3, and Alan H. Jobe2

1 Department of Pediatrics, University of Arizona and Steele Memorial Children's Research Center, Tucson, Arizona 85724; 2 Division of Pulmonary Biology, Children's Hospital Medical Center, Cincinnati, Ohio 45229; and 3 Department of Anatomy, University of California School of Veterinary Medicine, Davis, California 95616

The role of a lysosome fraction from rabbit type II cells in surfactant dipalmitoylphosphatidylcholine (DPPC) catabolism was investigated in vivo using radiolabeled DPPC and dihexadecylphosphatidylcholine (1,2-dihexadecyl-sn-glycero-3-phosphocholine; DEPC), a phospholipase A1- and A2-resistant analog of DPPC. Freshly isolated type II cells were gently disrupted by shearing, and lysosomes were isolated with Percoll density gradients (density range 1.0591-1.1457 g/ml). The lysosome fractions were relatively free of contaminating organelles as determined by electron microscopy and organelle marker enzymes. After intratracheal injection of rabbits with [3H]DPPC and [14C]DEPC associated with a trace amount of natural rabbit surfactant, the degradation-resistant DEPC accumulated 16-fold compared with DPPC in lysosome fractions at 15 h. Lysosomes can be isolated from freshly isolated type II cells, and lysosomes from type II cells are the primary catabolic organelle for alveolar surfactant DPPC following reuptake by type II cells in vivo.

lamellar bodies; surfactant metabolism; dipalmitoylphosphatidylcholine


This article has been cited by other articles:


Home page
 J. Lipid Res.Home page
A. B. Fisher, C. Dodia, S. I. Feinstein, and Y.-S. Ho
Altered lung phospholipid metabolism in mice with targeted deletion of lysosomal-type phospholipase A2
J. Lipid Res., June 1, 2005; 46(6): 1248 - 1256.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
B. W. Kramer, A. H. Jobe, and M. Ikegami
Exogenous surfactant changes the phenotype of alveolar macrophages in mice
Am J Physiol Lung Cell Mol Physiol, April 1, 2001; 280(4): L689 - L694.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. B. Fisher and C. Dodia
Lysosomal-type PLA2 and turnover of alveolar DPPC
Am J Physiol Lung Cell Mol Physiol, April 1, 2001; 280(4): L748 - L754.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
B. W. Kramer, M. Ikegami, and A. H. Jobe
Surfactant phospholipid catabolic rate is pool size dependent in mice
Am J Physiol Lung Cell Mol Physiol, November 1, 2000; 279(5): L842 - L848.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online