AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 278: L545-L551, 2000;
1040-0605/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (30)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cohen, P.
Right arrow Articles by Herrick, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cohen, P.
Right arrow Articles by Herrick, D. J.
Vol. 278, Issue 3, L545-L551, March 2000

IGFBP-3 mediates TGF-beta 1-induced cell growth in human airway smooth muscle cells

Pinchas Cohen1, Roopmathy Rajah1, Joel Rosenbloom2, and David J. Herrick2

1 Department of Pediatrics, Mattel Children's Hospital at University of California, Los Angeles, California 90095-1752; and 2 Department of Anatomy, University of Pennsylvania, Children's Hospital, Philadelphia, Pennsylvania 19104

Both insulin-like growth factor binding protein-3 (IGFBP-3) and transforming growth factor-beta (TGF-beta ) have been separately shown to have cell-specific growth-inhibiting or growth-potentiating effects. TGF-beta stimulates IGFBP-3 mRNA and peptide expression in several cell types, and TGF-beta -induced growth inhibition and apoptosis have been shown to be mediated through the induction of IGFBP-3. However, a link between the growth stimulatory effects of TGF-beta and IGFBP-3-induction has not been shown. In this study, we investigated the role of IGFBP-3 in mediating TGF-beta 1-induced cell growth using human airway smooth muscle (ASM) cells as our model. TGF-beta 1 (1 ng/ml) treatment induced a 10- to 20-fold increase in the levels of expression of IGFBP-3 mRNA and protein. Addition of either IGFBP-3 or TGF-beta 1 to the growth medium resulted in an approximately twofold increase in cell proliferation. Coincubation of ASM cells with IGFBP-3 antisense (but not sense) oligomers as well as with an IGFBP-3 neutralizing antibody (but not with control IgG) blocked the growth induced by TGF-beta 1 (P < 0.001). Several IGFBP-3-associated proteins were observed in ASM cell lysates, which may have a role in the cellular responses to IGFBP-3. These findings demonstrate that IGFBP-3 is capable of mediating the growth stimulatory effect of TGF-beta in ASM cells.

insulin-like growth factor binding protein; cell proliferation; receptor; transforming growth factor.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
M. Li, C. Li, Y.-h. Liu, Y. Xing, L. Hu, Z. Borok, K. Y.-C. Kwong, and P. Minoo
Mesodermal Deletion of Transforming Growth Factor-{beta} Receptor II Disrupts Lung Epithelial Morphogenesis: CROSS-TALK BETWEEN TGF-{beta} AND SONIC HEDGEHOG PATHWAYS
J. Biol. Chem., December 26, 2008; 283(52): 36257 - 36264.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
J. K. Bentley and M. B. Hershenson
Airway Smooth Muscle Growth in Asthma: Proliferation, Hypertrophy, and Migration
Proceedings of the ATS, January 1, 2008; 5(1): 89 - 96.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Xie, M. B. Sukkar, R. Issa, N. M. Khorasani, and K. F. Chung
Mechanisms of induction of airway smooth muscle hyperplasia by transforming growth factor-beta
Am J Physiol Lung Cell Mol Physiol, July 1, 2007; 293(1): L245 - L253.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Sturrock, T. P. Huecksteadt, K. Norman, K. Sanders, T. M. Murphy, P. Chitano, K. Wilson, J. R. Hoidal, and T. P. Kennedy
Nox4 mediates TGF-beta1-induced retinoblastoma protein phosphorylation, proliferation, and hypertrophy in human airway smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, June 1, 2007; 292(6): L1543 - L1555.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
Y. Bosse, C. Thompson, J. Stankova, and M. Rola-Pleszczynski
Fibroblast Growth Factor 2 and Transforming Growth Factor beta1 Synergism in Human Bronchial Smooth Muscle Cell Proliferation
Am. J. Respir. Cell Mol. Biol., June 1, 2006; 34(6): 746 - 753.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
K. F. Chung
The Role of Airway Smooth Muscle in the Pathogenesis of Airway Wall Remodeling in Chronic Obstructive Pulmonary Disease
Proceedings of the ATS, November 1, 2005; 2(4): 347 - 354.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
L O'Rear, L Longobardi, M Torello, B K Law, H L Moses, F Chiarelli, and A Spagnoli
Signaling cross-talk between IGF-binding protein-3 and transforming growth factor-{beta} in mesenchymal chondroprogenitor cell growth
J. Mol. Endocrinol., June 1, 2005; 34(3): 723 - 737.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Epidemiol. Biomarkers Prev.Home page
L. M. Morimoto, P. A. Newcomb, E. White, J. Bigler, and J. D. Potter
Insulin-like Growth Factor Polymorphisms and Colorectal Cancer Risk
Cancer Epidemiol. Biomarkers Prev., May 1, 2005; 14(5): 1204 - 1211.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Xie, M. B. Sukkar, R. Issa, U. Oltmanns, A. G. Nicholson, and K. F. Chung
Regulation of TGF-{beta}1-induced connective tissue growth factor expression in airway smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, January 1, 2005; 288(1): L68 - L76.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
T. Ikezoe, S. Tanosaki, U. Krug, B. Liu, P. Cohen, H. Taguchi, and H. P. Koeffler
Insulin-like growth factor binding protein-3 antagonizes the effects of retinoids in myeloid leukemia cells
Blood, July 1, 2004; 104(1): 237 - 242.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. L. Martin, S. M. Weenink, and R. C. Baxter
Insulin-like Growth Factor-binding Protein-3 Potentiates Epidermal Growth Factor Action in MCF-10A Mammary Epithelial Cells. INVOLVEMENT OF p44/42 AND p38 MITOGEN-ACTIVATED PROTEIN KINASES
J. Biol. Chem., January 24, 2003; 278(5): 2969 - 2976.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
J. K. Burgess, P. R. A. Johnson, Q. Ge, W. W. Au, M. H. Poniris, B. E. McParland, G. King, M. Roth, and J. L. Black
Expression of Connective Tissue Growth Factor in Asthmatic Airway Smooth Muscle Cells
Am. J. Respir. Crit. Care Med., January 1, 2003; 167(1): 71 - 77.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
W. A. Price, B. M. Moats-Staats, and A. D. Stiles
Pro- and Anti-inflammatory Cytokines Regulate Insulin-like Growth Factor Binding Protein Production by Fetal Rat Lung Fibroblasts
Am. J. Respir. Cell Mol. Biol., March 1, 2002; 26(3): 283 - 289.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Page, A. J. Ammit, J. L. Black, and C. L. Armour
Human mast cell and airway smooth muscle cell interactions: implications for asthma
Am J Physiol Lung Cell Mol Physiol, December 1, 2001; 281(6): L1313 - L1323.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online