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Am J Physiol Lung Cell Mol Physiol 278: L618-L624, 2000;
1040-0605/00 $5.00
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Vol. 278, Issue 3, L618-L624, March 2000

Fas cross-linking induces apoptosis in human airway smooth muscle cells

Kimm J. Hamann*, Joaquim E. Vieira*, Andrew J. Halayko, Delbert Dorscheid, Steven R. White, Sean M. Forsythe, Blanca Camoretti-Mercado, Klaus F. Rabe, and Julian Solway

Section of Pulmonary and Critical Care Medicine, Department of Medicine, University of Chicago, Chicago, Illinois 60637; and Krankenhaus Grosshansdorf, Grosshansdorf, Germany

Hypertrophy and hyperplasia lead to excess accumulation of smooth muscle in the airways of human asthmatic subjects. However, little is known about mechanisms that might counterbalance these processes, thereby limiting the quantity of smooth muscle in airways. Ligation of Fas on the surface of vascular smooth muscle cells and nonmuscle airway cells can lead to apoptotic cell death. We therefore tested the hypotheses that 1) human airway smooth muscle (HASM) expresses Fas, 2) Fas cross-linking induces apoptosis in these cells, and 3) tumor necrosis factor (TNF)-alpha potentiates Fas-mediated airway myocyte killing. Immunohistochemistry using CH-11 anti-Fas monoclonal IgM antibody revealed Fas expression in normal human bronchial smooth muscle in vivo. Flow cytometry using DX2 anti-Fas monoclonal IgG antibody revealed that passage 4 cultured HASM cells express surface Fas. Surface Fas decreased partially during prolonged serum deprivation of cultured HASM cells and was upregulated by TNF-alpha stimulation. Fas cross-linking with CH-11 antibody induced apoptosis in cultured HASM cells, and this effect was reduced by long-term serum deprivation and synergistically potentiated by concomitant TNF-alpha exposure. TNF-alpha did not induce substantial apoptosis in the absence of Fas cross-linking. These data represent the first demonstration that Fas is expressed on HASM and suggest a mechanism by which Fas-mediated apoptosis could act to oppose excess smooth muscle accumulation during airway remodeling in asthma.

asthma; airway remodeling; cytokines

*  K. J. Hamann and J. E. Vieira contributed equally to this work.




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