| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Medical Research Council Human Genetics Unit, Western General Hospital, and 3 Medical Genetics Section, Molecular Medicine Centre, Department of Medical Sciences, University of Edinburgh, Western General Hospital, Edinburgh EH4 2XU, United Kingdom; and 2 University of North Carolina Cystic Fibrosis Center, Chapel Hill, North Carolina 27599
The goal of this study was to develop a
primary culture model of differentiated murine tracheal epithelium.
When grown on semipermeable membranes at an air interface, dissociated
murine tracheal epithelial cells formed confluent polarized epithelia with high transepithelial resistances (~12
k
· cm2) that remained viable for up to 80 days.
Immunohistochemistry and light and electron microscopy demonstrated
that the cells were epithelial in nature (cytokeratin positive,
vimentin and 
-smooth muscle actin negative) and differentiated to
form ciliated and secretory cells from day 8 after seeding
onward. With RT-PCR, expression of the cystic fibrosis transmembrane
conductance regulator (Cftr) and murine 
-defensin
(Defb) genes was detected (Defb-1 was
constitutively expressed, whereas Defb-2 expression was
induced by exposure to lipopolysaccharide). Finally, Ussing chamber
experiments demonstrated an electrophysiological profile compatible
with functional amiloride-sensitive sodium channels and cAMP-stimulated
CFTR chloride channels. These data indicate that primary cultures of
murine tracheal epithelium have many characteristics similar to those of murine tracheal epithelium in vivo. This method will facilitate the
establishment of primary cultures of airway epithelium from transgenic
mouse models of human diseases.
cystic fibrosis; cystic fibrosis transmembrane conductance regulator; defensins; airway surface liquid; airway epithelium
This article has been cited by other articles:
|
|
 |
|
  S. Y. Kassim, S. A. Gharib, B. H. Mecham, T. P. Birkland, W. C. Parks, and J. K. McGuire Individual Matrix Metalloproteinases Control Distinct Transcriptional Responses in Airway Epithelial Cells Infected with Pseudomonas aeruginosa Infect. Immun., December 1, 2007; 75(12): 5640 - 5650. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Liu, M. Luo, L. Zhang, W. Ding, Z. Yan, and J. F. Engelhardt Bioelectric Properties of Chloride Channels in Human, Pig, Ferret, and Mouse Airway Epithelia Am. J. Respir. Cell Mol. Biol., March 1, 2007; 36(3): 313 - 323. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Dejima, S. H. Randell, M. J. Stutts, B. A. Senior, and R. C. Boucher Potential Role of Abnormal Ion Transport in the Pathogenesis of Chronic Sinusitis Arch Otolaryngol Head Neck Surg, December 1, 2006; 132(12): 1352 - 1362. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. R. Grubb, T. D. Rogers, P. C. Diggs, R. C. Boucher, and L. E. Ostrowski Culture of murine nasal epithelia: model for cystic fibrosis Am J Physiol Lung Cell Mol Physiol, February 1, 2006; 290(2): L270 - L277. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Liu, Z. Yan, M. Luo, and J. F. Engelhardt Species-Specific Differences in Mouse and Human Airway Epithelial Biology of Recombinant Adeno-Associated Virus Transduction Am. J. Respir. Cell Mol. Biol., January 1, 2006; 34(1): 56 - 64. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. McMichael, A. I. Maxwell, K. Hayashi, K. Taylor, W. A. Wallace, J. R. Govan, J. R. Dorin, and J.-M. Sallenave Antimicrobial Activity of Murine Lung Cells against Staphylococcus aureus Is Increased In Vitro and In Vivo after Elafin Gene Transfer Infect. Immun., June 1, 2005; 73(6): 3609 - 3617. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. MacPherson, P. Keir, S. Webb, K. Samuel, S. Boyle, W. Bickmore, L. Forrester, and J. Dorin Bone marrow-derived SP cells can contribute to the respiratory tract of mice in vivo J. Cell Sci., June 1, 2005; 118(11): 2441 - 2450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. G. Schoch, A. Lori, K. A. Burns, T. Eldred, J. C. Olsen, and S. H. Randell A subset of mouse tracheal epithelial basal cells generates large colonies in vitro Am J Physiol Lung Cell Mol Physiol, April 1, 2004; 286(4): L631 - L642. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. You, E. J. Richer, T. Huang, and S. L. Brody Growth and differentiation of mouse tracheal epithelial cells: selection of a proliferative population Am J Physiol Lung Cell Mol Physiol, December 1, 2002; 283(6): L1315 - L1321. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
