AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 279: L825-L834, 2000;
1040-0605/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (6)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Papakonstantinou, E.
Right arrow Articles by Roth, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Papakonstantinou, E.
Right arrow Articles by Roth, M.
Vol. 279, Issue 5, L825-L834, November 2000

Hypoxia modifies the effect of PDGF on glycosaminoglycan synthesis by primary human lung cells

Eleni Papakonstantinou1, George Karakiulakis1, Michael Tamm2, André P. Perruchoud2, and Michael Roth2

1 Department of Pharmacology, School of Medicine, Aristotle University, 54006 Thessaloniki, Greece; and 2 Departments of Research and Internal Medicine, University Hospital Basel, 4031 Basel, Switzerland

Hypoxia, a consequence of interstitial lung diseases, may lead to secondary pulmonary hypertension and pulmonary vascular remodeling. Hypoxia induces activation and proliferation of lung cells and enhances the deposition of extracellular matrix including glycosaminoglycans (GAGs). To elucidate the cell biological mechanisms underlying the development of secondary pulmonary hypertension, we studied the effect of hypoxia on GAG synthesis by human lung cells. GAG synthesis was measured by incorporation of [3H]glucosamine; GAGs were isolated, purified, and characterized with GAG-degrading enzymes. Fibroblasts and vascular smooth muscle cells (VSMCs) synthesized hyaluronic acid, heparan sulfate, and chondroitin sulfates, whereas dermatan sulfate was found only in fibroblasts. Hypoxia did not influence the size or charge of the individual GAGs. However, hypoxia inhibited platelet-derived growth factor-induced [3H]glucosamine incorporation in secreted GAGs, especially hyaluronic acid, in VSMCs. In contrast, it stimulated GAG secretion, specifically heparan sulfate, by fibroblasts. Our results indicate that hypoxia induces modifications in GAG synthesis by human lung VSMCs and fibroblasts that may be correlated to pathophysiological manifestations in lung diseases causing hypoxia.

platelet-derived growth factor; primary lung fibroblasts; primary lung vascular smooth muscle cells; pulmonary fibrosis; pulmonary hypertension


This article has been cited by other articles:


Home page
 Eur Respir JHome page
E. Papakonstantinou, F. M. Kouri, G. Karakiulakis, I. Klagas, and O. Eickelberg
Increased hyaluronic acid content in idiopathic pulmonary arterial hypertension
Eur. Respir. J., December 1, 2008; 32(6): 1504 - 1512.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Aytekin, S. A. A. Comhair, C. de la Motte, S. K. Bandyopadhyay, C. F. Farver, V. C. Hascall, S. C. Erzurum, and R. A. Dweik
High levels of hyaluronan in idiopathic pulmonary arterial hypertension
Am J Physiol Lung Cell Mol Physiol, November 1, 2008; 295(5): L789 - L799.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
E. Papakonstantinou, M. Roth, M. Tamm, O. Eickelberg, A. P. Perruchoud, and G. Karakiulakis
Hypoxia Differentially Enhances the Effects of Transforming Growth Factor-beta Isoforms on the Synthesis and Secretion of Glycosaminoglycans by Human Lung Fibroblasts
J. Pharmacol. Exp. Ther., June 1, 2002; 301(3): 830 - 837.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online