AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 280: L482-L492, 2001;
1040-0605/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (16)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kube, D.
Right arrow Articles by Davis, P. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kube, D.
Right arrow Articles by Davis, P. B.
Vol. 280, Issue 3, L482-L492, March 2001

Terminal sialylation is altered in airway cells with impaired CFTR-mediated chloride transport

Dianne Kube, Lynn Adams, Aura Perez, and Pamela B. Davis

Department of Pediatrics, Case Western Reserve University at Rainbow Babies and Children's Hospital, Cleveland, Ohio 44106

Reduced terminal sialylation at the surface of airway epithelial cells from patients with cystic fibrosis may predispose them to bacterial infection. To determine whether a lack of chloride transport or misprocessing of mutant cystic fibrosis transmembrane conductance regulator (CFTR) is critical for the alterations in glycosylation, we studied a normal human tracheal epithelial cell line (9/HTEo-) transfected with the regulatory (R) domain of CFTR, which blocks CFTR-mediated chloride transport; Delta F508 CFTR, which is misprocessed, wild-type CFTR; or empty vector. Reduced cAMP-stimulated chloride transport is seen in the R domain and Delta F508 transfectants. These two cell lines had consistent, significantly reduced binding of elderberry bark lectin, which recognizes terminal sialic acid in the alpha -2,6 configuration. Binding of other lectins, including Maakia amurensis lectin, which recognizes sialic acid in the alpha -2,3 configuration, was comparable in all cell lines. Because the cell surface change occurred in R domain-transfected cells, which continue to express wild-type CFTR, it cannot be related entirely to misprocessed or overexpressed CFTR. It is associated most closely with reduced CFTR activity.

cystic fibrosis; cystic fibrosis transmembrane conductance regulator; Delta F508; regulatory domain; lectins; fluorescent imaging; immunogold labeling; 3-(2,4-dinitroanilino)-3'-amino-N-methyldipropylamine


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Perez, A. M. van Heeckeren, D. Nichols, S. Gupta, J. F. Eastman, and P. B. Davis
Peroxisome proliferator-activated receptor-{gamma} in cystic fibrosis lung epithelium
Am J Physiol Lung Cell Mol Physiol, August 1, 2008; 295(2): L303 - L313.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
B. L Schulz, A. J Sloane, L. J Robinson, S. S Prasad, R. A Lindner, M. Robinson, P. T Bye, D. W Nielson, J. L Harry, N. H Packer, et al.
Glycosylation of sputum mucins is altered in cystic fibrosis patients
Glycobiology, July 1, 2007; 17(7): 698 - 712.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Perez, A. C. Issler, C. U. Cotton, T. J. Kelley, A. S. Verkman, and P. B. Davis
CFTR inhibition mimics the cystic fibrosis inflammatory profile
Am J Physiol Lung Cell Mol Physiol, February 1, 2007; 292(2): L383 - L395.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
K. Kunzelmann and B. McMorran
First Encounter: How Pathogens Compromise Epithelial Transport
Physiology, October 1, 2004; 19(5): 240 - 244.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
A. Muir, G. Soong, S. Sokol, B. Reddy, M. I. Gomez, A. van Heeckeren, and A. Prince
Toll-Like Receptors in Normal and Cystic Fibrosis Airway Epithelial Cells
Am. J. Respir. Cell Mol. Biol., June 1, 2004; 30(6): 777 - 783.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
J. B. Lyczak, C. L. Cannon, and G. B. Pier
Lung Infections Associated with Cystic Fibrosis
Clin. Microbiol. Rev., April 1, 2002; 15(2): 194 - 222.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
T. J. Kelley, H. L. Elmer, and D. A. Corey
Reduced Smad3 Protein Expression and Altered Transforming Growth Factor-beta 1-Mediated Signaling in Cystic Fibrosis Epithelial Cells
Am. J. Respir. Cell Mol. Biol., December 1, 2001; 25(6): 732 - 738.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. F. Poschet, J. C. Boucher, L. Tatterson, J. Skidmore, R. W. Van Dyke, and V. Deretic
Molecular basis for defective glycosylation and Pseudomonas pathogenesis in cystic fibrosis lung
PNAS, November 20, 2001; 98(24): 13972 - 13977.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. Kube, U. Sontich, D. Fletcher, and P. B. Davis
Proinflammatory cytokine responses to P. aeruginosa infection in human airway epithelial cell lines
Am J Physiol Lung Cell Mol Physiol, March 1, 2001; 280(3): L493 - L502.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online