AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 281: L1078-L1087, 2001;
1040-0605/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (15)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Al-Jamal, R.
Right arrow Articles by Ludwig, M. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Al-Jamal, R.
Right arrow Articles by Ludwig, M. S.
Vol. 281, Issue 5, L1078-L1087, November 2001

Changes in proteoglycans and lung tissue mechanics during excessive mechanical ventilation in rats

Rehab Al-Jamal and Mara S. Ludwig

Meakins-Christie Laboratories, Royal Victoria Hospital, McGill University, Montreal, Quebec H2X 2P2, Canada

Excessive mechanical ventilation results in changes in lung tissue mechanics. We hypothesized that changes in tissue properties might be related to changes in the extracellular matrix component proteoglycans (PGs). The effect of different ventilation regimens on lung tissue mechanics and PGs was examined in an in vivo rat model. Animals were anesthetized, tracheostomized, and ventilated at a tidal volume of 8 (VT8), 20, or 30 (VT30) ml/kg, positive end-expiratory pressure of 0 (PEEP0) or 1.5 (PEEP1.5) cmH2O, and frequency of 1.5 Hz for 2 h. The constant-phase model was used to derive airway resistance, tissue elastance, and tissue damping. After physiological measurements, one lung was frozen for immunohistochemistry and the other was reserved for PG extraction and Western blotting. After 2 h of mechanical ventilation, tissue elastance and damping were significantly increased in rats ventilated at VT30PEEP0 compared with control rats (ventilated at VT8PEEP1.5). Versican, basement membrane heparan sulfate PG, and biglycan were all increased in rat lungs ventilated at VT30PEEP0 compared with control rats. At VT30PEEP0, heparan sulfate PG and versican staining became prominent in the alveolar wall and airspace; biglycan was mostly localized in the airway wall. These data demonstrate that alterations in lung tissue mechanics with excessive mechanical ventilation are accompanied by changes in all classes of extracellular matrix PG.

tissue resistance; viscoelasticity; extracellular matrix


This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
A. Moriondo, P. Pelosi, A. Passi, M. Viola, C. Marcozzi, P. Severgnini, V. Ottani, M. Quaranta, and D. Negrini
Proteoglycan fragmentation and respiratory mechanics in mechanically ventilated healthy rats
J Appl Physiol, September 1, 2007; 103(3): 747 - 756.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
M. S. Ludwig
Proteoglycans and pathophysiology
J Appl Physiol, September 1, 2007; 103(3): 735 - 736.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. Okutani, B. Han, M. Mura, T. K. Waddell, S. Keshavjee, and M. Liu
High-volume ventilation induces pentraxin 3 expression in multiple acute lung injury models in rats
Am J Physiol Lung Cell Mol Physiol, January 1, 2007; 292(1): L144 - L153.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
B. Chen, Y. Wen, Z. Zhang, Y. Guo, J. A. Warrington, and M. L. Polan
Microarray analysis of differentially expressed genes in vaginal tissues from women with stress urinary incontinence compared with asymptomatic women
Hum. Reprod., January 1, 2006; 21(1): 22 - 29.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
K.-J. Bai, A. P. Spicer, M. M. Mascarenhas, L. Yu, C. D. Ochoa, H. G. Garg, and D. A. Quinn
The Role of Hyaluronan Synthase 3 in Ventilator-induced Lung Injury
Am. J. Respir. Crit. Care Med., July 1, 2005; 172(1): 92 - 98.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. Kornecki, S. Tsuchida, H. K. Ondiveeran, D. Engelberts, H. Frndova, A. K. Tanswell, M. Post, C. McKerlie, J. Belik, A. Fox-Robichaud, et al.
Lung Development and Susceptibility to Ventilator-induced Lung Injury
Am. J. Respir. Crit. Care Med., April 1, 2005; 171(7): 743 - 752.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
S. Ito, E. P. Ingenito, S. P. Arold, H. Parameswaran, N. T. Tgavalekos, K. R. Lutchen, and B. Suki
Tissue heterogeneity in the mouse lung: effects of elastase treatment
J Appl Physiol, July 1, 2004; 97(1): 204 - 212.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. M. Mascarenhas, R. M. Day, C. D. Ochoa, W.-I. Choi, L. Yu, B. Ouyang, H. G. Garg, C. A. Hales, and D. A. Quinn
Low Molecular Weight Hyaluronan from Stretched Lung Enhances Interleukin-8 Expression
Am. J. Respir. Cell Mol. Biol., January 1, 2004; 30(1): 51 - 60.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
O. U. Gurkan, C. O'Donnell, R. Brower, E. Ruckdeschel, and P. M. Becker
Differential effects of mechanical ventilatory strategy on lung injury and systemic organ inflammation in mice
Am J Physiol Lung Cell Mol Physiol, September 1, 2003; 285(3): L710 - L718.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online