AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 282: L197-L206, 2002;
1040-0605/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (39)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Brown, J. K.
Right arrow Articles by Hall, I. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brown, J. K.
Right arrow Articles by Hall, I. P.
Vol. 282, Issue 2, L197-L206, February 2002

EDITORIAL FOCUS
Tryptase's potent mitogenic effects in human airway smooth muscle cells are via nonproteolytic actions

James K. Brown1,2, Cary A. Jones1, Leeann A. Rooney1, George H. Caughey2, and Ian P. Hall3

1 Pulmonary and Critical Care Medicine Section, Medical Service, Department of Veterans Affairs Medical Center, San Francisco 94121; 2 Cardiovascular Research Institute and Department of Medicine, University of California, San Francisco, CA 94143-0911; and 3 Division of Therapeutics, University Hospital, Nottingham NG7 2UH, United Kingdom

We reported previously that mast cell tryptase is a growth factor for dog tracheal smooth muscle cells. The goals of our current experiments were to determine if tryptase also is mitogenic in cultured human airway smooth muscle cells, to compare its strength as a growth factor with that of other mitogenic serine proteases, and to determine whether its proteolytic actions are required for mitogenesis. Highly purified preparations of human lung beta -tryptase (1-30 nM) caused dose-dependent increases in DNA synthesis in human airway smooth muscle cells. Maximum tryptase-induced increases in DNA synthesis far exceeded those occurring in response to coagulation cascade proteases, such as thrombin, factor Xa, or factor XII, or to other mast cell proteases, such as chymase or mastin. Irreversibly abolishing tryptase's catalytic activity did not alter its effects on increases in DNA synthesis. We conclude that beta -tryptase is a potent mitogenic serine protease in cultured human airway smooth muscle cells. However, its growth stimulatory effects in these cells occur predominantly via nonproteolytic actions.

mast cell proteases; serine proteases; airway smooth muscle hyperplasia; noncatalytic effects of proteases


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
P. Rastogi, D. M. Young, and J. McHowat
Tryptase activates calcium-independent phospholipase A2 and releases PGE2 in airway epithelial cells
Am J Physiol Lung Cell Mol Physiol, November 1, 2008; 295(5): L925 - L932.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
C. S. Mullan, M. Riley, D. Clarke, A. Tatler, A. Sutcliffe, A. J. Knox, and L. Pang
{beta}-Tryptase Regulates IL-8 Expression in Airway Smooth Muscle Cells by a PAR-2-Independent Mechanism
Am. J. Respir. Cell Mol. Biol., May 1, 2008; 38(5): 600 - 608.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
J. K. Bentley and M. B. Hershenson
Airway Smooth Muscle Growth in Asthma: Proliferation, Hypertrophy, and Migration
Proceedings of the ATS, January 1, 2008; 5(1): 89 - 96.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
P. Bradding
Mast cell regulation of airway smooth muscle function in asthma
Eur. Respir. J., May 1, 2007; 29(5): 827 - 830.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. K. Brown, M. D. Hollenberg, and C. A. Jones
Tryptase activates phosphatidylinositol 3-kinases proteolytically independently from proteinase-activated receptor-2 in cultured dog airway smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, February 1, 2006; 290(2): L259 - L269.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
A M Sutcliffe and A J Knox
Muscling into cystic fibrosis airways
Thorax, March 1, 2005; 60(3): 181 - 182.
[Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
A. Masamune, K. Kikuta, M. Satoh, N. Suzuki, and T. Shimosegawa
Protease-Activated Receptor-2-Mediated Proliferation and Collagen Production of Rat Pancreatic Stellate Cells
J. Pharmacol. Exp. Ther., February 1, 2005; 312(2): 651 - 658.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. Chokki, S. Yamamura, H. Eguchi, T. Masegi, H. Horiuchi, H. Tanabe, T. Kamimura, and S. Yasuoka
Human Airway Trypsin-Like Protease Increases Mucin Gene Expression in Airway Epithelial Cells
Am. J. Respir. Cell Mol. Biol., April 1, 2004; 30(4): 470 - 478.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. S. Chambers, J. L. Black, Q. Ge, S. M. Carlin, W. W. Au, M. Poniris, J. Thompson, P. R. Johnson, and J. K. Burgess
PAR-2 activation, PGE2, and COX-2 in human asthmatic and nonasthmatic airway smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, September 1, 2003; 285(3): L619 - L627.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. L. Lazaar, M. I. Plotnick, U. Kucich, I. Crichton, S. Lotfi, S. K. P. Das, S. Kane, J. Rosenbloom, R. A. Panettieri Jr., N. M. Schechter, et al.
Mast Cell Chymase Modifies Cell-Matrix Interactions and Inhibits Mitogen-Induced Proliferation of Human Airway Smooth Muscle Cells
J. Immunol., July 15, 2002; 169(2): 1014 - 1020.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
W. M. Abraham
Tryptase: potential role in airway inflammation and remodeling
Am J Physiol Lung Cell Mol Physiol, February 1, 2002; 282(2): L193 - L196.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online