AJP - Lung AJP citation statistics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 282: L631-L641, 2002. First published October 5, 2001; doi:10.1152/ajplung.00085.2001
1040-0605/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/4/L631    most recent
00085.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (39)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Itani, O. A.
Right arrow Articles by Thomas, C. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Itani, O. A.
Right arrow Articles by Thomas, C. P.
Vol. 282, Issue 4, L631-L641, April 2002

SPECIAL TOPIC
Alveolar Epithelial Ion and Fluid Transport
Glucocorticoid-stimulated lung epithelial Na+ transport is associated with regulated ENaC and sgk1 expression

Omar A. Itani1, Scott D. Auerbach1, Russell F. Husted1, Kenneth A. Volk1, Shana Ageloff2, Mark A. Knepper2, John B. Stokes1,3, and Christie P. Thomas1,3

1 Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242; 3 Veterans Affairs Medical Center, Iowa City, Iowa 52246; and 2 Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892

H441 cells, a bronchiolar epithelial cell line, develop a glucocorticoid-regulated amiloride-sensitive Na+ transport pathway on permeable supports (R. Sayegh, S. D. Auerbach, X. Li, R. Loftus, R. Husted, J. B. Stokes, and C. P. Thomas. J Biol Chem 274: 12431-12437, 1999). To understand its molecular basis, we examined the effect of glucocorticoids (GC) on epithelial Na+ channel (ENaC)-alpha , -beta , and -gamma and sgk1 expression and determined the biophysical properties of Na+ channels in these cells. GC stimulated the expression of ENac-alpha , -beta , and -gamma and sgk1 mRNA, with the first effect seen by 1 h. These effects were abolished by actinomycin D, but not by cycloheximide, indicating a direct stimulatory effect on ENaC and sgk1 mRNA synthesis. The GC effect on transcription of ENaC-alpha mRNA was accompanied by a significant increase in ENaC-alpha protein levels. GC also stimulated ENaC-alpha , -beta , and -gamma and sgk1 mRNA expression in A549 cells, an alveolar type II cell line. To determine the biophysical properties of the Na+ channel, single-channel currents were recorded from cell-attached H441 membranes. An Na+-selective channel with slow kinetics and a slope conductance of 10.8 pS was noted, properties similar to ENaC-alpha , -beta , and -gamma expressed in Xenopus laevis oocytes. These experiments indicate that amiloride-sensitive Na+ transport is mediated through classic ENaC channels in human lung epithelia and that GC-regulated Na+ transport is accompanied by increased transcription of each of the component subunits and sgk1.

epithelial sodium channel; amiloride; short-circuit current; patch clamp; adenosine 3',5'-cyclic monophosphate; corticosteroids; airway epithelia; alveolar type II cells


This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
H. Sheng, T. Sun, B. Cong, P. He, Y. Zhang, J. Yan, C. Lu, and X. Ni
Corticotropin-releasing hormone stimulates SGK-1 kinase expression in cultured hippocampal neurons via CRH-R1
Am J Physiol Endocrinol Metab, October 1, 2008; 295(4): E938 - E946.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
V. Shlyonsky, A. Goolaerts, F. Mies, and R. Naeije
Electrophysiological Characterization of Rat Type II Pneumocytes in Situ
Am. J. Respir. Cell Mol. Biol., July 1, 2008; 39(1): 36 - 44.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
G. Otulakowski, W. Duan, S. Gandhi, and H. O'Brodovich
Steroid and Oxygen Effects on eIF4F Complex, mTOR, and ENaC Translation in Fetal Lung Epithelia
Am. J. Respir. Cell Mol. Biol., October 1, 2007; 37(4): 457 - 466.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
R. F. Husted, K. A. Volk, R. D. Sigmund, and J. B. Stokes
Discordant effects of corticosteroids and expression of subunits on ENaC activity
Am J Physiol Renal Physiol, September 1, 2007; 293(3): F813 - F820.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. M. Myerburg, J. D. Latoche, E. E. McKenna, L. P. Stabile, J. S. Siegfried, C. A. Feghali-Bostwick, and J. M. Pilewski
Hepatocyte growth factor and other fibroblast secretions modulate the phenotype of human bronchial epithelial cells
Am J Physiol Lung Cell Mol Physiol, June 1, 2007; 292(6): L1352 - L1360.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. R. Quesnell, X. Han, and B. D. Schultz
Glucocorticoids stimulate ENaC upregulation in bovine mammary epithelium
Am J Physiol Cell Physiol, May 1, 2007; 292(5): C1739 - C1745.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Dagenais, R. Frechette, M.-E. Clermont, C. Masse, A. Prive, E. Brochiero, and Y. Berthiaume
Dexamethasone inhibits the action of TNF on ENaC expression and activity
Am J Physiol Lung Cell Mol Physiol, December 1, 2006; 291(6): L1220 - L1231.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
F. Lang, C. Bohmer, M. Palmada, G. Seebohm, N. Strutz-Seebohm, and V. Vallon
(Patho)physiological Significance of the Serum- and Glucocorticoid-Inducible Kinase Isoforms.
Physiol Rev, October 1, 2006; 86(4): 1151 - 1178.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
G. Otulakowski, B. Rafii, M. Harris, and H. O'Brodovich
Oxygen and Glucocorticoids Modulate {alpha}ENaC mRNA Translation in Fetal Distal Lung Epithelium
Am. J. Respir. Cell Mol. Biol., February 1, 2006; 34(2): 204 - 212.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
S. R. Pondugula, N. N. Raveendran, Z. Ergonul, Y. Deng, J. Chen, J. D. Sanneman, L. G. Palmer, and D. C. Marcus
Glucocorticoid regulation of genes in the amiloride-sensitive sodium transport pathway by semicircular canal duct epithelium of neonatal rat
Physiol Genomics, January 12, 2006; 24(2): 114 - 123.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
M. Quinkler, I. J. Bujalska, K. Kaur, C. U. Onyimba, S. Buhner, B. Allolio, S. V. Hughes, M. Hewison, and P. M. Stewart
Androgen Receptor-Mediated Regulation of the {alpha}-Subunit of the Epithelial Sodium Channel in Human Kidney
Hypertension, October 1, 2005; 46(4): 787 - 798.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. P. Thomas, J. R. Campbell, P. J. Wright, and R. F. Husted
cAMP-stimulated Na+ transport in H441 distal lung epithelial cells: role of PKA, phosphatidylinositol 3-kinase, and sgk1
Am J Physiol Lung Cell Mol Physiol, October 1, 2004; 287(4): L843 - L851.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
C. P. Thomas, R. W. Loftus, and K. Z. Liu
AVP-induced VIT32 gene expression in collecting duct cells occurs via trans-activation of a CRE in the 5'-flanking region of the VIT32 gene
Am J Physiol Renal Physiol, September 1, 2004; 287(3): F460 - F468.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. J. Ramminger, K. Richard, S. K. Inglis, S. C. Land, R. E. Olver, and S. M. Wilson
A regulated apical Na+ conductance in dexamethasone-treated H441 airway epithelial cells
Am J Physiol Lung Cell Mol Physiol, August 1, 2004; 287(2): L411 - L419.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
M. T. Clunes, A. G. Butt, and S. M. Wilson
A glucocorticoid-induced Na+ conductance in human airway epithelial cells identified by perforated patch recording
J. Physiol., June 15, 2004; 557(3): 809 - 819.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
T. J. Cole, N. M. Solomon, R. Van Driel, J. A. Monk, D. Bird, S. J. Richardson, R. J. Dilley, and S. B. Hooper
Altered Epithelial Cell Proportions in the Fetal Lung of Glucocorticoid Receptor Null Mice
Am. J. Respir. Cell Mol. Biol., May 1, 2004; 30(5): 613 - 619.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. B. Mustafa, R. J. DiGeronimo, J. A. Petershack, J. L. Alcorn, and S. R. Seidner
Postnatal glucocorticoids induce {alpha}-ENaC formation and regulate glucocorticoid receptors in the preterm rabbit lung
Am J Physiol Lung Cell Mol Physiol, January 1, 2004; 286(1): L73 - L80.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
B. C. Willis, K.-J. Kim, X. Li, J. Liebler, E. D. Crandall, and Z. Borok
Modulation of ion conductance and active transport by TGF-{beta}1 in alveolar epithelial cell monolayers
Am J Physiol Lung Cell Mol Physiol, December 1, 2003; 285(6): L1192 - L1200.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
O. A. Weisz and J. P. Johnson
Noncoordinate regulation of ENaC: paradigm lost?
Am J Physiol Renal Physiol, November 1, 2003; 285(5): F833 - F842.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
O. A. Itani, J. R. Campbell, J. Herrero, P. M. Snyder, and C. P. Thomas
Alternate promoters and variable splicing lead to hNedd4-2 isoforms with a C2 domain and varying number of WW domains
Am J Physiol Renal Physiol, November 1, 2003; 285(5): F916 - F929.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
O. A. Itani, K. L. Cornish, K. Z. Liu, and C. P. Thomas
Cycloheximide increases glucocorticoid-stimulated alpha -ENaC mRNA in collecting duct cells by p38 MAPK-dependent pathway
Am J Physiol Renal Physiol, April 1, 2003; 284(4): F778 - F787.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. Jovanovic, R. M. Crawford, H. J. Ranki, and A. Jovanovic
Large Conductance Ca2+-Activated K+ Channels Sense Acute Changes in Oxygen Tension in Alveolar Epithelial Cells
Am. J. Respir. Cell Mol. Biol., March 1, 2003; 28(3): 363 - 372.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
U. H. Thome, I. C. Davis, S. V. Nguyen, B. J. Shelton, and S. Matalon
Modulation of sodium transport in fetal alveolar epithelial cells by oxygen and corticosterone
Am J Physiol Lung Cell Mol Physiol, February 1, 2003; 284(2): L376 - L385.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
O. A. Itani, K. Z. Liu, K. L. Cornish, J. R. Campbell, and C. P. Thomas
Glucocorticoids stimulate human sgk1 gene expression by activation of a GRE in its 5'-flanking region
Am J Physiol Endocrinol Metab, November 1, 2002; 283(5): E971 - E979.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. A. Matthay
Editorial: Alveolar Epithelial Ion and Fluid Transport: Regulation of ion and fluid transport across the distal pulmonary epithelia: new insights
Am J Physiol Lung Cell Mol Physiol, April 1, 2002; 282(4): L595 - L598.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online