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Am J Physiol Lung Cell Mol Physiol 282: L1339-L1348, 2002. First published January 12, 2002; doi:10.1152/ajplung.00392.2001
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Vol. 282, Issue 6, L1339-L1348, June 2002

Human bronchial epithelial cells express PAR-2 with different sensitivity to thermolysin

Joachim J. Ubl1, Zoryana V. Grishina1,2, Tatiana K. Sukhomlin1,3, Tobias Welte4, Fariba Sedehizade1, and Georg Reiser1

1 Institut für Neurobiochemie, 4 Abteilung für Pneumologie der Medizinischen Fakultät der Otto-von-Guericke-Universität Magdeburg, 39120 Magdeburg, Germany; 2 A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, 119899 Moscow; and 3 Research Centre of Molecular Diagnostics and Therapy, 113149 Moscow, Russia

Protease-activated receptor-2 (PAR-2) plays a role in inflammatory reactions in airway physiology. Proteases cleaving the extracellular NH2 terminus of receptors activate or inactivate PAR, thus possessing a therapeutic potential. Using RT-PCR and immunocytochemistry, we show PAR-2 in human airway epithelial cell lines human bronchial epithelial (HBE) and A549. Functional expression of PAR-2 was confirmed by Ca2+ imaging studies using the receptor agonist protease trypsin. The effect was abolished by soybean trypsin inhibitor and mimicked by the specific PAR-2 peptide agonist SLIGKV. Amplitude and duration of PAR-2-elicited Ca2+ response in HBE and A549 cells depend on concentration and time of agonist superfusion. The response is partially pertussis toxin (PTX) insensitive, abolished by the phospholipase C inhibitor U-73122, and diminished by the inositol 1,4,5-trisphosphate receptor antagonist 2-aminoethoxydiphenyl borate. Cathepsin G altered neither the resting Ca2+ level nor PAR-2-elicited Ca2+ response. Thermolysin, a prototypic bacterial metalloprotease, induced a dose-dependent Ca2+ response in HBE, but not A549, cells. In both cell lines, thermolysin abolished the response to a subsequent trypsin challenge but not to SLIGKV. Thus different epithelial cell types express different PAR-2 with identical responses to physiological stimuli (trypsin, SLIGKV) but different sensitivity to modifying proteases, such as thermolysin.

calcium signaling; cathepsin G; trypsin; protease-activated receptor-2


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