AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 283: L596-L603, 2002. First published April 5, 2002; doi:10.1152/ajplung.00446.2001
1040-0605/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/3/L596    most recent
00446.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (19)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Samb, A.
Right arrow Articles by Boczkowski, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Samb, A.
Right arrow Articles by Boczkowski, J.
Vol. 283, Issue 3, L596-L603, September 2002

Heme oxygenase modulates oxidant-signaled airway smooth muscle contractility: role of bilirubin

Abdoulaye Samb1,*, Camille Taillé1,*, Abdelhamid Almolki1, Jérôme Mégret1, James M. Staddon2, Michel Aubier1, and Jorge Boczkowski1

1 Institut National pour la Santé et la Recherche Médicale, Unité 408, Faculté de Médecine Xavier Bichat, 75018 Paris, France; and 2 Eisai London Research Laboratories, University College London, London WC1E 6BT, United Kingdom

Reactive oxygen species (ROS) increase the contractile response of airway smooth muscle (ASM). Heme oxygenase (HO) catabolizes heme to the powerful antioxidant bilirubin. Because HO is expressed in the airways, we investigated its effects on ASM contractility and ROS production in guinea pig trachea. HO expression was higher in the epithelium than in tracheal smooth muscle. Incubation of tracheal rings (TR) with the HO inhibitor tin protoporphyrin (SnPP IX) or the HO substrate hemin increased and decreased, respectively, ASM contractile response to carbamylcholine. The effect of hemin was reversed by SnPP and mimicked by the antioxidants superoxide dismutase (SOD) and catalase. Hemin significantly reduced the effect of carbamylcholine in rings treated with the guanylate cyclase inhibitor 1H-[1,2,4]oxadiazolo-[4,3-a]quinoxalin-1-one (ODQ), compared with ODQ-treated rings without hemin incubation, suggesting that the CO-guanosine 3',5'-cyclic monophosphate pathway was not involved in the control of tracheal reactivity. SnPP and hemin increased and decreased ROS production by TR by 18 and 38%, respectively. Bilirubin (100 pM) significantly decreased TR contractility and ROS production. Hemin, bilirubin, and SOD/catalase decreased phosphorylation of the contractile protein myosin light chain, whereas SnPP significantly augmented it. These data suggest that modulation of the redox status by HO and, moreover, by bilirubin modulates ASM contractility by modulating levels of phosphorylated myosin light chain.

phosphorylation; myosin light chain; reactive oxygen species

* A. Samb and C. Taillé contributed equally to this work.




This article has been cited by other articles:


Home page
 Am. J. Respir. Crit. Care Med.Home page
T. Sato, M. Takeno, K. Honma, H. Yamauchi, Y. Saito, T. Sasaki, H. Morikubo, Y. Nagashima, S. Takagi, K. Yamanaka, et al.
Heme Oxygenase-1, a Potential Biomarker of Chronic Silicosis, Attenuates Silica-induced Lung Injury
Am. J. Respir. Crit. Care Med., October 15, 2006; 174(8): 906 - 914.
[Abstract] [Full Text] [PDF]

Home page
 J. Med. Genet.Home page
A Guenegou, B Leynaert, J Benessiano, I Pin, P Demoly, F Neukirch, J Boczkowski, and M Aubier
Association of lung function decline with the heme oxygenase-1 gene promoter microsatellite polymorphism in a general population sample. Results from the European Community Respiratory Health Survey (ECRHS), France.
J. Med. Genet., August 1, 2006; 43(8): e43 - e43.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
D. Morse and A. M. K. Choi
Heme Oxygenase-1: From Bench to Bedside
Am. J. Respir. Crit. Care Med., September 15, 2005; 172(6): 660 - 670.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
A. Pflueger, A. J. Croatt, T. E. Peterson, L. A. Smith, L. V. d'Uscio, Z. S. Katusic, and K. A. Nath
The hyperbilirubinemic Gunn rat is resistant to the pressor effects of angiotensin II
Am J Physiol Renal Physiol, March 1, 2005; 288(3): F552 - F558.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Almolki, C. Taille, G. F. Martin, P. J. Jose, C. Zedda, M. Conti, J. Megret, D. Henin, M. Aubier, and J. Boczkowski
Heme oxygenase attenuates allergen-induced airway inflammation and hyperreactivity in guinea pigs
Am J Physiol Lung Cell Mol Physiol, July 1, 2004; 287(1): L26 - L34.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
S. J. Gibbons and G. Farrugia
The role of carbon monoxide in the gastrointestinal tract
J. Physiol., April 15, 2004; 556(2): 325 - 336.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online