AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 283: L707-L716, 2002. First published May 17, 2002; doi:10.1152/ajplung.00024.2002
1040-0605/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/4/L707    most recent
00024.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (13)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, Y. D.
Right arrow Articles by Patel, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, Y. D.
Right arrow Articles by Patel, J. M.
Vol. 283, Issue 4, L707-L716, October 2002

Activation of multiple signaling modules is critical in angiotensin IV-induced lung endothelial cell proliferation

Yong D. Li1, Edward R. Block1,2, and Jawaharlal M. Patel1,2

1 Department of Medicine, University of Florida College of Medicine; and 2 Research Service, Malcom Randall Department of Veterans Affairs Medical Center, Gainesville, Florida 32608-1197

Signaling events involving angiotensin IV (ANG IV)-mediated pulmonary artery endothelial cell (PAEC) proliferation were examined. ANG IV significantly increased upstream phosphatidylinositide (PI) 3-kinase (PI3K), PI-dependent kinase-1 (PDK-1), extracellular signal-related kinases (ERK1/2), and protein kinase B-alpha /Akt (PKB-alpha ) activities, as well as downstream p70 ribosomal S6 kinase (p70S6K) activities and/or phosphorylation of these proteins. ANG IV also significantly increased 5-bromo-2'-deoxy-uridine incorporation into newly synthesized DNA in a concentration- and time-dependent manner. Pretreatment of cells with wortmannin and LY-294002, inhibitors of PI3K, or rapamycin, an inhibitor of the mammalian target of rapamycin kinase and p70S6K, diminished the ANG IV-mediated activation of PDK-1 and PKB-alpha as well as phosphorylation of p70S6K. Although an inhibitor of mitogen-activated protein kinase kinase, PD-98059, but not rapamycin, blocked ANG IV-induced phosphorylation of ERK1/2, both PD-98059 and rapamycin independently caused partial reduction in ANG IV-mediated cell proliferation. However, simultaneous treatment with PD-98059 and rapamycin resulted in total inhibition of ANG IV-induced cell proliferation. These results demonstrate that ANG IV-induced DNA synthesis is regulated in a coordinated fashion involving multiple signaling modules in PAEC.

p70 S6 kinase; protein kinase B; phosphatidylinositol 3-kinase


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
K. Kotlo, S. Shukla, U. Tawar, R. A. Skidgel, and R. S. Danziger
Aminopeptidase N reduces basolateral Na+-K+-ATPase in proximal tubule cells
Am J Physiol Renal Physiol, October 1, 2007; 293(4): F1047 - F1053.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
X. C. Li, D. J. Campbell, M. Ohishi, S. Yuan, and J. L. Zhuo
AT1 receptor-activated signaling mediates angiotensin IV-induced renal cortical vasoconstriction in rats
Am J Physiol Renal Physiol, May 1, 2006; 290(5): F1024 - F1033.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
V. Esteban, M. Ruperez, E. Sanchez-Lopez, J. Rodriguez-Vita, O. Lorenzo, H. Demaegdt, P. Vanderheyden, J. Egido, and M. Ruiz-Ortega
Angiotensin IV Activates the Nuclear Transcription Factor-{kappa}B and Related Proinflammatory Genes in Vascular Smooth Muscle Cells
Circ. Res., May 13, 2005; 96(9): 965 - 973.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
T. Watanabe, T. A. Barker, and B. C. Berk
Angiotensin II and the Endothelium: Diverse Signals and Effects
Hypertension, February 1, 2005; 45(2): 163 - 169.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
N. Lochard, G. Thibault, D. W. Silversides, R. M. Touyz, and T. L. Reudelhuber
Chronic Production of Angiotensin IV in the Brain Leads to Hypertension That Is Reversible With an Angiotensin II AT1 Receptor Antagonist
Circ. Res., June 11, 2004; 94(11): 1451 - 1457.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online