AJP - Lung AJP: Cell Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 283: L922-L931, 2002. First published May 24, 2002; doi:10.1152/ajplung.00014.2002
1040-0605/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/5/L922    most recent
00014.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schroedl, C.
Right arrow Articles by Chandel, N. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schroedl, C.
Right arrow Articles by Chandel, N. S.
Vol. 283, Issue 5, L922-L931, November 2002

EDITORIAL FOCUS
Hypoxic but not anoxic stabilization of HIF-1alpha requires mitochondrial reactive oxygen species

Clara Schroedl, David S. McClintock, G. R. Scott Budinger, and Navdeep S. Chandel

Division of Pulmonary and Critical Care Medicine, Department of Medicine, Northwestern University Medical School, Chicago, Illinois 60611

The molecular mechanisms by which cells detect hypoxia (1.5% O2), resulting in the stabilization of hypoxia-inducible factor 1alpha (HIF-1alpha ) protein remain unclear. One model proposes that mitochondrial generation of reactive oxygen species is required to stabilize HIF-1alpha protein. Primary evidence for this model comes from the observation that cells treated with complex I inhibitors, such as rotenone, or cells that lack mitochondrial DNA (rho 0-cells) fail to generate reactive oxygen species or stabilize HIF-1alpha protein in response to hypoxia. In the present study, we investigated the role of mitochondria in regulating HIF-1alpha protein stabilization under anoxia (0% O2). Wild-type A549 and HT1080 cells stabilized HIF-1alpha protein in response to hypoxia and anoxia. The rho 0-A549 cells and rho 0-HT1080 cells failed to accumulate HIF-1alpha protein in response to hypoxia. However, both rho 0-A549 and rho 0-HT1080 were able to stabilize HIF-1alpha protein levels in response to anoxia. Rotenone inhibited hypoxic, but not anoxic, stabilization of HIF-1alpha protein. These results indicate that a functional electron transport chain is required for hypoxic but not anoxic stabilization of HIF-1alpha protein.

rho zero; rotenone; complex I


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
T. Presley, K. Vedam, M. Velayutham, J. L. Zweier, and G. Ilangovan
Activation of Hsp90-eNOS and increased NO generation attenuate respiration of hypoxia-treated endothelial cells
Am J Physiol Cell Physiol, November 1, 2008; 295(5): C1281 - C1291.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. A. Qutub and A. S. Popel
Reactive Oxygen Species Regulate Hypoxia-Inducible Factor 1{alpha} Differentially in Cancer and Ischemia
Mol. Cell. Biol., August 15, 2008; 28(16): 5106 - 5119.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
E. Hervouet, A. Cizkova, J. Demont, A. Vojtiskova, P. Pecina, N. L.W. Franssen-van Hal, J. Keijer, H. Simonnet, R. Ivanek, S. Kmoch, et al.
HIF and reactive oxygen species regulate oxidative phosphorylation in cancer
Carcinogenesis, August 1, 2008; 29(8): 1528 - 1537.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
E. H. Ruder, T. J. Hartman, J. Blumberg, and M. B. Goldman
Oxidative stress and antioxidants: exposure and impact on female fertility
Hum. Reprod. Update, June 4, 2008; (2008) dmn011v1.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. T. Brown and C. A. Nurse
Induction of HIF-2{alpha} is dependent on mitochondrial O2 consumption in an O2-sensitive adrenomedullary chromaffin cell line
Am J Physiol Cell Physiol, June 1, 2008; 294(6): C1305 - C1312.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. Kaewpila, S. Venkataraman, G. R. Buettner, and L. W. Oberley
Manganese Superoxide Dismutase Modulates Hypoxia-Inducible Factor-1{alpha} Induction via Superoxide
Cancer Res., April 15, 2008; 68(8): 2781 - 2788.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
A. J. Glassford, P. Yue, A. Y. Sheikh, H. J. Chun, S. Zarafshar, D. A. Chan, G. M. Reaven, T. Quertermous, and P. S. Tsao
HIF-1 regulates hypoxia- and insulin-induced expression of apelin in adipocytes
Am J Physiol Endocrinol Metab, December 1, 2007; 293(6): E1590 - E1596.
[Abstract] [Full Text] [PDF]

Home page
 The OncologistHome page
L. J. Costa and H. A. Drabkin
Renal Cell Carcinoma: New Developments in Molecular Biology and Potential for Targeted Therapies
Oncologist, December 1, 2007; 12(12): 1404 - 1415.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. M. Guidot, H. G. Folkesson, L. Jain, J. I. Sznajder, J.-F. Pittet, and M. A. Matthay
Integrating acute lung injury and regulation of alveolar fluid clearance
Am J Physiol Lung Cell Mol Physiol, September 1, 2006; 291(3): L301 - L306.
[Abstract] [Full Text] [PDF]

Home page
 Exp PhysiolHome page
R. D. Guzy and P. T. Schumacker
Oxygen sensing by mitochondria at complex III: the paradox of increased reactive oxygen species during hypoxia
Exp Physiol, September 1, 2006; 91(5): 807 - 819.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. A. Qutub and A. S. Popel
A computational model of intracellular oxygen sensing by hypoxia-inducible factor HIF1{alpha}.
J. Cell Sci., August 15, 2006; 119(Pt 16): 3467 - 3480.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
T. E. Machen
Innate immune response in CF airway epithelia: hyperinflammatory?
Am J Physiol Cell Physiol, August 1, 2006; 291(2): C218 - C230.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
A. P. Comellas, L. A. Dada, E. Lecuona, L. M. Pesce, N. S. Chandel, N. Quesada, G. R. S. Budinger, G. J. Strous, A. Ciechanover, and J. I. Sznajder
Hypoxia-Mediated Degradation of Na,K-ATPase via Mitochondrial Reactive Oxygen Species and the Ubiquitin-Conjugating System
Circ. Res., May 26, 2006; 98(10): 1314 - 1322.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
M. Jain and J. I. Sznajder
Effects of Hypoxia on the Alveolar Epithelium
Proceedings of the ATS, October 1, 2005; 2(3): 202 - 205.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
K. Doege, S. Heine, I. Jensen, W. Jelkmann, and E. Metzen
Inhibition of mitochondrial respiration elevates oxygen concentration but leaves regulation of hypoxia-inducible factor (HIF) intact
Blood, October 1, 2005; 106(7): 2311 - 2317.
[Abstract] [Full Text] [PDF]

Home page
 Sci SignalHome page
B. M. Emerling and N. S. Chandel
Oxygen Sensing: Getting Pumped by Sterols
Sci. Signal., June 21, 2005; 2005(289): pe30 - pe30.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
B. M. Emerling, L. C. Platanias, E. Black, A. R. Nebreda, R. J. Davis, and N. S. Chandel
Mitochondrial Reactive Oxygen Species Activation of p38 Mitogen-Activated Protein Kinase Is Required for Hypoxia Signaling
Mol. Cell. Biol., June 15, 2005; 25(12): 4853 - 4862.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Y. Gong and F. H. Agani
Oligomycin inhibits HIF-1{alpha} expression in hypoxic tumor cells
Am J Physiol Cell Physiol, May 1, 2005; 288(5): C1023 - C1029.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
K. D. Mansfield, M. C. Simon, and B. Keith
Hypoxic reduction in cellular glutathione levels requires mitochondrial reactive oxygen species
J Appl Physiol, October 1, 2004; 97(4): 1358 - 1366.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
A. J. Giaccia, M. C. Simon, and R. Johnson
The biology of hypoxia: the role of oxygen sensing in development, normal function, and disease
Genes & Dev., September 15, 2004; 18(18): 2183 - 2194.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
R. K. Bruick
Oxygen sensing in the hypoxic response pathway: regulation of the hypoxia-inducible transcription factor
Genes & Dev., November 1, 2003; 17(21): 2614 - 2623.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
P. T. Schumacker
Hypoxia, anoxia, and O2 sensing: the search continues
Am J Physiol Lung Cell Mol Physiol, November 1, 2002; 283(5): L918 - L921.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online