| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Division of Pulmonary Biology, 2 Graduate Program for Molecular and Developmental Biology, and 3 Division of Human Genetics, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio 45229-3039
Regulation of the surfactant protein B gene (SP-B) is developmentally controlled and highly tissue specific. To elucidate the SP-B gene temporal/spatial expression pattern in lung development at the transcriptional level, a transgenic mouse model line carrying the human SP-B (hSP-B) 1.5-kb 5'-flanking regulatory region and the lacZ gene was established. Expression of hSP-B 1.5-kb lacZ gene started at the onset of lung formation [embryonic day 9 (E9)] and was restricted to epithelial cells throughout prenatal and postnatal lung development. In the adult lung, hSP-B 1.5-kb lacZ gene expression was restricted to bronchiolar and alveolar type II epithelial cells. In lung explant culturing studies, the hSP-B 1.5-kb lacZ gene was highly expressed in newly formed epithelial tubules during the respiratory branching process. In a second transgenic mouse line, an enhancer region, which binds to thyroid transcription factor-1, retinoic acid receptor, signal transducers and activators of transcription 3, and nuclear receptor coactivators (SRC-1, ACTR, TIF2, and CBP/p300), was deleted from the hSP-B 1.5-kb lacZ gene. The deletion abolished hSP-B lacZ gene expression in bronchiolar epithelial cells and significantly reduced its expression level in alveolar type II epithelial cells in transgenic mice.
lung development; thyroid transcription factor-1; retinoic acid receptor; signal transducers and activators of transcription 3; human surfactant protein B; lung branching morphogenesis; lung explant; alveolar type II
This article has been cited by other articles:
|
|
 |
|
  B. Zhou, D. K. Ann, P. Flodby, P. Minoo, J. M. Liebler, E. D. Crandall, and Z. Borok Rat aquaporin-5 4.3-kb 5'-flanking region differentially regulates expression in salivary gland and lung in vivo Am J Physiol Cell Physiol, July 1, 2008; 295(1): C111 - C120. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. H. Thomas, P. Meyn, and N. Suttorp Single nucleotide polymorphism in 5'-flanking region reduces transcription of surfactant protein B gene in H441 cells Am J Physiol Lung Cell Mol Physiol, September 1, 2006; 291(3): L386 - L390. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. W. Glasser, S. K. Eszterhas, E. A. Detmer, M. D. Maxfield, and T. R. Korfhagen The murine SP-C promoter directs type II cell-specific expression in transgenic mice Am J Physiol Lung Cell Mol Physiol, April 1, 2005; 288(4): L625 - L632. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Yan and H. Du Alveolus formation: what have we learned from genetic studies? J Appl Physiol, October 1, 2004; 97(4): 1543 - 1548. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
