HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 290/6/L1267    most recent 00515.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (17) Citing Articles via Google Scholar Google Scholar Articles by Yang, X.-R. Articles by Sham, J. S. K. Search for Related Content PubMed PubMed Citation Articles by Yang, X.-R. Articles by Sham, J. S. K.

Functional expression of transient receptor potential melastatin- and vanilloid-related channels in pulmonary arterial and aortic smooth muscle

¹Division of Pulmonary and Critical Care Medicine, Johns Hopkins School of Medicine, Baltimore, Maryland; and ²Department of Physiology and Pathophysiology, Fujian Medical University, Fuxhou, Fujian, People's Republic of China

Submitted 7 December 2005 ; accepted in final form 29 December 2005

Transient receptor potential melastatin- (TRPM) and vanilloid-related (TRPV) channels are nonselective cation channels pertinent to diverse physiological functions. Multiple TRPM and TRPV channel subtypes have been identified and cloned in different tissues. However, their information in vascular tissue is scant. In this study, we sought to identify TRPM and TRPV channel subtypes expressed in rat deendothelialized intralobar pulmonary arteries (PAs) and aorta. With RT-PCR, mRNA of TRPM2, TRPM3, TRPM4, TRPM7, and TRPM8 of TRPM family and TRPV1, TRPV2, TRPV3, and TRPV4 of TRPV family were detected in both PAs and aorta. Quantitative real-time RT-PCR showed that TRPM8 and TRPV4 were the most abundantly expressed TRPM and TRPV subtypes, respectively. Moreover, Western blot analysis verified expression of TRPM2, TRPM8, TRPV1, and TRPV4 proteins in both types of vascular tissue. To examine the functional activities of these channels, we monitored intracellular Ca²⁺ transients ([Ca²⁺]_i) in pulmonary arterial smooth muscle cells (PASMCs) and aortic smooth muscle cells (ASMCs). The TRPM8 agonist menthol (300 µM) and the TRPV4 agonist 4-phorbol 12,13-didecanoate (1 µM) evoked significant increases in [Ca²⁺]_i in PASMCs and ASMCs. These Ca²⁺ responses were abolished in the absence of extracellular Ca²⁺ or the presence of 300 µM Ni²⁺ but were unaffected by 1 µM nifedipine, suggesting Ca²⁺ influx via nonselective cation channels. Hence, for the first time, our results indicate that multiple functional TRPM and TRPV channels are coexpressed in rat intralobar PAs and aorta. These novel Ca²⁺ entry pathways may play important roles in the regulation of pulmonary and systemic circulation.

transient receptor potential channels; calcium signaling; nonselective cation channels

This article has been cited by other articles:

				R. N. Willette, W. Bao, S. Nerurkar, T.-l. Yue, C. P. Doe, G. Stankus, G. H. Turner, H. Ju, H. Thomas, C. E. Fishman, et al. Systemic Activation of the Transient Receptor Potential Vanilloid Subtype 4 Channel Causes Endothelial Failure and Circulatory Collapse: Part 2 J. Pharmacol. Exp. Ther., August 1, 2008; 326(2): 443 - 452. [Abstract] [Full Text] [PDF]

				B. Zhong and D. H. Wang N-oleoyldopamine, a novel endogenous capsaicin-like lipid, protects the heart against ischemia-reperfusion injury via activation of TRPV1 Am J Physiol Heart Circ Physiol, August 1, 2008; 295(2): H728 - H735. [Abstract] [Full Text] [PDF]

				I. N. Bratz, G. M. Dick, J. D. Tune, J. M. Edwards, Z. P. Neeb, U. D. Dincer, and M. Sturek Impaired capsaicin-induced relaxation of coronary arteries in a porcine model of the metabolic syndrome Am J Physiol Heart Circ Physiol, June 1, 2008; 294(6): H2489 - H2496. [Abstract] [Full Text] [PDF]

				S. Ito, H. Kume, K. Naruse, M. Kondo, N. Takeda, S. Iwata, Y. Hasegawa, and M. Sokabe A Novel Ca2+ Influx Pathway Activated by Mechanical Stretch in Human Airway Smooth Muscle Cells Am. J. Respir. Cell Mol. Biol., April 1, 2008; 38(4): 407 - 413. [Abstract] [Full Text] [PDF]

				C. Nassenstein, K. Kwong, T. Taylor-Clark, M. Kollarik, D. M. MacGlashan, A. Braun, and B. J. Undem Expression and function of the ion channel TRPA1 in vagal afferent nerves innervating mouse lungs J. Physiol., March 15, 2008; 586(6): 1595 - 1604. [Abstract] [Full Text] [PDF]

				R. M. Touyz Transient receptor potential melastatin 6 and 7 channels, magnesium transport, and vascular biology: implications in hypertension Am J Physiol Heart Circ Physiol, March 1, 2008; 294(3): H1103 - H1118. [Abstract] [Full Text] [PDF]

				M.-J. Lin, X.-R. Yang, Y.-N. Cao, and J. S. K. Sham Hydrogen peroxide-induced Ca2+ mobilization in pulmonary arterial smooth muscle cells Am J Physiol Lung Cell Mol Physiol, June 1, 2007; 292(6): L1598 - L1608. [Abstract] [Full Text] [PDF]

				S. Earley, S. V. Straub, and J. E. Brayden Protein kinase C regulates vascular myogenic tone through activation of TRPM4 Am J Physiol Heart Circ Physiol, June 1, 2007; 292(6): H2613 - H2622. [Abstract] [Full Text] [PDF]

				D. L. Cioffi The Skinny on TRPV1 Circ. Res., April 13, 2007; 100(7): 934 - 936. [Full Text] [PDF]

				R. Inoue, L. J. Jensen, J. Shi, H. Morita, M. Nishida, A. Honda, and Y. Ito Transient Receptor Potential Channels in Cardiovascular Function and Disease Circ. Res., July 21, 2006; 99(2): 119 - 131. [Abstract] [Full Text] [PDF]

				S. Earley Molecular Diversity of Receptor Operated Channels in Vascular Smooth Muscle: A Role for Heteromultimeric TRP Channels? Circ. Res., June 23, 2006; 98(12): 1462 - 1464. [Full Text] [PDF]