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GM-CSF receptor expression and signaling is decreased in lungs of ethanol-fed rats

¹Atlanta Veterans Affairs Medical Center, ²Department of Medicine, Atlanta; and ³Department of Physiology, Emory University School of Medicine, Atlanta, Georgia

Submitted 19 April 2006 ; accepted in final form 20 July 2006

Alcohol abuse dramatically increases the risk of acute lung injury. In an experimental rat model of ethanol-mediated susceptibility to lung injury, recombinant granulocyte/macrophage colony-stimulating factor (GM-CSF) restored alveolar epithelial barrier function both in vitro and in vivo, even during acute endotoxemia. These findings suggested that the alveolar epithelium, which secretes GM-CSF into the airway where it is required for alveolar macrophage maturation, likewise responds to GM-CSF priming in a receptor-mediated manner. In this study we determined that both the GM-CSF receptor - and -subunits (GM-CSFR and GM-CSFR) are expressed throughout the rat airway epithelium and that this expression was significantly decreased in the alveolar epithelium following chronic ethanol ingestion (6 wk). In parallel, PU.1, the master transcription factor for GM-CSF signaling in hematopoietic cells, is also expressed in alveolar epithelial cells, and ethanol ingestion likewise decreased PU.1 protein expression and nuclear binding in the alveolar epithelium. Finally, GM-CSF signaling as reflected by PU.1 expression and nuclear binding was restored with recombinant GM-CSF treatment in vitro. We conclude that chronic ethanol ingestion decreases GM-CSF receptor expression and signaling in the lung epithelium. Consequently, we speculate that dampening of GM-CSF stimulation of the alveolar epithelium is responsible at least in part for the diverse functional defects that characterize the alcoholic lung and could be a therapeutic target in acute lung injury.

acute respiratory distress syndrome; signal transduction; type II cells; transcription factor; alcohol abuse; granulocyte/macrophage colony-stimulating factor

This article has been cited by other articles:

				M. Koval, X. Fan, A. L. Fernandez, L. N. Johnson, P. C. Joshi, and D. M. Guidot Effects of Alcohol and GM-CSF on Alveolar Tight Junctions Proceedings of the ATS, April 15, 2008; 5(3): 368 - 369. [Full Text] [PDF]

				P. C. Joshi and D. M. Guidot The alcoholic lung: epidemiology, pathophysiology, and potential therapies Am J Physiol Lung Cell Mol Physiol, April 1, 2007; 292(4): L813 - L823. [Abstract] [Full Text] [PDF]