HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 292/5/L1052    most recent 00249.2006v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (5) Citing Articles via Google Scholar Google Scholar Articles by Tagaram, H. R. S. Articles by Floros, J. Search for Related Content PubMed PubMed Citation Articles by Tagaram, H. R. S. Articles by Floros, J.

EDITORIAL FOCUS

Characterization of a human surfactant protein A1 (SP-A1) gene-specific antibody; SP-A1 content variation among individuals of varying age and pulmonary health

Departments of ¹Cellular and Molecular Physiology, ²Pediatrics, ³Obstetrics and Gynecology, and ⁴Health Evaluation Sciences, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania; and ⁵Department of Pulmonary Critical Care Medicine, Cleveland Clinic Foundation, Cleveland, Ohio

Submitted 29 June 2006 ; accepted in final form 22 December 2006

The human surfactant protein A (SP-A) locus consists of two functional genes (SP-A1, SP-A2) with gene-specific products exhibiting qualitative and quantitative differences. The aim here was twofold: 1) generate SP-A1 gene-specific antibody, and 2) use this to assess gene-specific SP-A content in the bronchoalveolar lavage fluid (BALF). An SP-A1-specific polyclonal antibody (hSP-A1_Ab^68-88_Col) was raised in chicken, and its specificity was determined by immunoblot and ELISA using mammalian Chinese hamster ovary (CHO) cell-expressed SP-A1 and SP-A2 variants and by immunofluorescence with stably transfected CHO cell lines expressing SP-A1 or SP-A2 variants. SP-A1 content was evaluated according to age and lung status. A gradual decrease (P < 0.05) in SP-A1/SP-A ratio was observed in healthy subjects (HS) with increased age, although no significant change was observed in total SP-A content among age groups. Total SP-A and SP-A1 content differed significantly between alveolar proteinosis (AP) patients and HS, with no significant difference observed in SP-A1/SP-A ratio between AP and HS. The cystic fibrosis (CF) ratio was significantly higher compared with AP, HS, and noncystic fibrosis (NCF), even though SP-A1 and total SP-A were decreased in CF compared with most of the other groups. The ratio was higher in culture-positive vs. culture-negative samples from CF and NCF (P = 0.031). A trend of an increased ratio was observed in culture-positive CF (0.590 ± 0.10) compared with culture-positive NCF (0.368 ± 0.085). In summary, we developed and characterized an SP-A1 gene-specific antibody and used it to identify gene-specific SP-A content in BALFs as a function of age and lung health.

alveolar proteinosis; bronchoalveolar lavage fluid; cystic fibrosis; peptide antibody; surfactant protein A

This article has been cited by other articles:

				A. N. Mikerov, T. M. Umstead, X. Gan, W. Huang, X. Guo, G. Wang, D. S. Phelps, and J. Floros Impact of ozone exposure on the phagocytic activity of human surfactant protein A (SP-A) and SP-A variants Am J Physiol Lung Cell Mol Physiol, January 1, 2008; 294(1): L121 - L130. [Abstract] [Full Text] [PDF]