HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 293/1/L142    most recent 00434.2006v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (2) Citing Articles via Google Scholar Google Scholar Articles by Choo-Wing, R. Articles by Bhandari, V. Search for Related Content PubMed PubMed Citation Articles by Choo-Wing, R. Articles by Bhandari, V.

Developmental differences in the responses of IL-6 and IL-13 transgenic mice exposed to hyperoxia

¹Department of Pediatrics, Division of Perinatal Medicine, ²Department of Internal Medicine, Section of Pulmonary and Critical Care Medicine, and ³Department of Pathology, Yale University School of Medicine, New Haven, Connecticut

Submitted 2 November 2006 ; accepted in final form 26 March 2007

Our previous work has shown that adult mice with overexpression of IL-6 and IL-13 in the lung have enhanced survival in hyperoxia associated with reduced hyperoxia-induced lung injury and cell death. We hypothesized that there are developmental differences in these responses in the adult vs. the newborn (NB) animal, and these responses have clinical relevance in the human NB. We compared the responses to 100% O₂ of NB IL-6 and IL-13 transgenic mice with wild-type littermate controls by evaluating mortality, lung tissue TUNEL staining, and mRNA expression using RT-PCR. We used ELISA to measure IL-6 levels in tracheal aspirates from human neonates. Our results show that, in contrast to the cytoprotective effects in mature mice, IL-6 caused significantly increased mortality, DNA injury, caspases, cell death regulator and angiogenic factor expression in hyperoxia in the NB. Furthermore, tracheal aspirate levels of IL-6 were significantly increased in premature neonates with respiratory distress syndrome who had an adverse outcome (bronchopulmonary dysplasia/death). In contrast to the protective effects in adults, there was no survival advantage to the NB IL-13 mice in hyperoxia. These findings imply that caution should be exercised in extrapolating results from the adult to the NB.

cytokines; lung; newborn

This article has been cited by other articles:

				Q. Mao, S. Gundavarapu, C. Patel, A. Tsai, F. I. Luks, and M. E. De Paepe The Fas System Confers Protection against Alveolar Disruption in Hyperoxia-Exposed Newborn Mice Am. J. Respir. Cell Mol. Biol., December 1, 2008; 39(6): 717 - 729. [Abstract] [Full Text] [PDF]

				V. Bhandari, R. Choo-Wing, C. G. Lee, K. Yusuf, J. H. Nedrelow, N. Ambalavanan, H. Malkus, R. J. Homer, and J. A. Elias Developmental Regulation of NO-Mediated VEGF-Induced Effects in the Lung Am. J. Respir. Cell Mol. Biol., October 1, 2008; 39(4): 420 - 430. [Abstract] [Full Text] [PDF]

				P. M. Lavoie, C. Pham, and K. L. Jang Heritability of Bronchopulmonary Dysplasia, Defined According to the Consensus Statement of the National Institutes of Health Pediatrics, September 1, 2008; 122(3): 479 - 485. [Abstract] [Full Text] [PDF]